摘要
目的探讨肠源性内毒素血症对慢性实验性肝损伤的影响及防治对策。方法将Wistar大鼠随机分为3组:正常对照组、慢性肝损伤组及双利肝治疗组(双利肝组),每组10只。采用CCl4复合因素复制慢性肝损伤模型,4周末处死动物,取血检测血浆内毒素(ET),血清丙氨酸氨基转移酶(ALT)、γ-干扰素(IFN-γ)、白介素-2(IL-2)和白介素-4(IL-4),同时观察肝脏组织学变化。结果①CCl4所致的慢性肝损伤组与正常大鼠组相比,血浆ET、血清ALTI、L-4水平明显增高(P<0.05),血清IL-2、IFN-γ水平明显降低(P<0.05)。②与CCl4所致慢性肝损伤组相比,双利肝组血浆ET、血清ALTI、L-4水平明显下降(P<0.05),血清IL-2、IFN-γ水平明显增高(P<0.05)。③组织学观察:经双利肝治疗后肝脏组织学明显改善。结论CCl4所致慢性肝损伤大鼠存在肠源性内毒素血症,后者可能导致细胞免疫功能紊乱。经双利肝治疗后血浆内毒素水平明显下降,Th1/Th2比例得到调整,肝细胞损伤减轻。
Objective To study the effect of intestinal endotoxemia on chronic experimental liver injury and its therapeutic measures, Methods Rats were divided into 3 groups randomly： normal group, chronic liver injury group and shuangligan （DD） group （each, n = 10）. The rat model of chronic hepatic injury was made by subcutaneous injections of CCl4 and dietetic factors for 4 weeks. Blood was taken from abdominal aorta to determine the plasma ET, ALT, IL-2, IFN-γ and IL-4,and observe the changes of liver histology, Results （1）Compared with normal group, ET and ALT levels increased significantly in chronic liver injury group （P〈0.05）, IL-2 and IFN-γ levels were lower （P〈0.05）, while IL-4 level was higher（P〈 0.05）. （2）Compared to chronic liver injury group, levels of ET,ALT and IL-4 in DD group decreased, and IFN-γ and IL-2 increased. （3）The liver histologically iraproved after DD treatment, Conclusion The rats with chronic experimental hepatic group caused by CCl4 have intestinal endotoxemia which may lead to cellular immunologic function disorder, including Th1/Th2 imbalance. Following DD therapy, the ET level is significantly decreased and Th1/Th2 ratio is adjusted.
出处
《山西医科大学学报》
CAS
2006年第9期906-908,共3页
Journal of Shanxi Medical University
基金
山西省卫生厅科研基金资助项目(200205)
关键词
肝炎
药物性
慢性
内毒素血症
大鼠
hepatitis, chronic, drug-induced
endotoxemia
rats
