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N-乙酰-L-半胱氨酸对胎儿卵巢组织体外培养的影响 被引量:3

Effects of N-acetyl-L-cysteine on the fetal ovarian tissues cultured in vitro
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摘要 目的:探讨硫醇类抗氧化剂N-乙酰-L-半胱氨酸(n-acetyl—1-cysteine,NAC)对胎儿卵巢组织体外培养效果的影响。方法:取中期引产的20~28周死亡女胎的卵巢,分别在含NAC25、50、100mmol/L(实验组)和不合NAC(对照组)的培养液内培养0,9d,采用液相平衡竞争放射免疫分析法检测各组卵巢组织在不同的培养时间雌二醇(estrogen,E2)分泌量;观察培养后各组卵巢卵泡的形态学变化。结果:培养后卵泡可进一步发育,各期卵泡构成比实验组与未培养组、对照组比较差异均有统计学意义(P〈0.05),而3个不同剂量实验组两两之间在卵泡构成比上差异无统计学意义(P〉0.05);胎儿卵巢组织经体外培养后能够分泌E2,E2分泌量实验组和对照组之间差异有统计学意义(P〈0.05),随着培养时间的延长B分泌量显著增加(P〈0.05);而在添加不同剂量NAC的3个实验组,两两比较差异则无统计学意义(P〉0.05)。结论:在培养液中添加NAC后,B分泌量增加,各期卵泡的形态得以维持并能进一步发育,为人胎儿卵巢培养后移植提供了实验依据。 Objective: To investigate the effects of antioxidant N-acetyl-L-cysteine(NAC) on the fetal ovarian tissues cultured in vitro. Methods: Fetal ovaries of 20 to 28 weeks were cultured for 0 to 9 days in 4 NAC groups : 25 mmol/L, 50 mmol/L, 100 mmol/L and 0 mmol/L. The concentrations of estrogen in the culture medium were measured by radio-immunoassay and the morphologic changes of the cultured ovalian tissues were observed. Results: The proportion of matured follicles of different stages in the 3 experimental groups were significantly different compared with the control group (P 〈 0.05) and the estrogen output in both control and experimental groups were significantly increased with the time prolonged(P 〈 0.05), however, the differences among the experimental groups were not significant (P 〉 0.05); the estrogen output of the fetal ovarian tissues in the 3 experimental groups were significantly increased than that in the control group ( P 〈 0.05), however, the differences among the experimental groups were not significant ( P 〉 0.05). Conclusion: N-acetyl-L-cysteine can increase the estrogen output and the proportion of mature follicles in the fetal ovarian tissues cultured in vitro.
出处 《山东大学学报(医学版)》 CAS 北大核心 2006年第11期1128-1131,共4页 Journal of Shandong University:Health Sciences
基金 山东省自然科学基金资助课题(Y2003C05)
关键词 卵巢 胎儿 N-乙酰-L-半胱氨酸 Ovary Fetus N-acetyl-L-cysteine
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  • 1刘晓红,郑英,孙丽君.胎儿与成人卵巢免疫原性的比较[J].河南医科大学学报,2000,35(1):10-12. 被引量:15
  • 2Otala M,Erkkila K,Tuuri T,et al.Cell death and its suppression in human ovarian tissue culture[J].Mole Hum Reprod,2002,8(3):228-236. 被引量:1
  • 3De Flora S,Izzotti A,D'Agostini F,et al.Mechanism of N-acetyl-cysteine in the prevention of DNA damage and cancer,with special reference to smoking-felatde end-points[J].Carcinogenesis,2001,22 (7):999-1 001. 被引量:1
  • 4谭丽,郑英.胎儿卵巢组织培养与冷冻保存[J].河南医科大学学报,1993,28(3):210-213. 被引量:5
  • 5Delneste Y,Jeannin P.Thiols prevent Fas(CD-95)-mediated T cell apoptosis by down-regulating membrane Fas expression[J].Eur J Immunol,1996,26(12):2 981-2 988. 被引量:1
  • 6Erkkila K,Hirvonen V.NAC inhibits apoptosis in human male germ cells in vitro[J].J Clin Endocrinol Metab,1998,83(7):2 523-2 531. 被引量:1
  • 7Tilly JL,Tilly KI.Inhibitors of oxidative stress mimic the ability of follicle-stimulating hormone to suppress apoptosis in cultured rat ovarian follicles[ J].Endocrinology,1995,136(1):242-252. 被引量:1
  • 8Parasassi T,Brunelli R,Bracci-Laudiero L,et al.Differentiation of normal and cancer cells induced by sulfhydryl reduction:biochemical and molecular mechanisms[J].Cell Death Differ,2005,12(10):1 285-1 296. 被引量:1

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  • 1覃波霖,陈学进,施振旦,李万利,田允波.小鼠卵巢冷冻移植后卵泡发育和卵母细胞成熟的研究[J].生理学报,2006,58(1):41-46. 被引量:12
  • 2邓敏,车小群.羊膜细胞培养液对胎儿卵巢组织雌二醇分泌的实验研究[J].齐齐哈尔医学院学报,2006,27(5):526-526. 被引量:1
  • 3Gougeno A. Dynamics of follicular growth in the human : a model from preliminary results [J]. Hum Reprod, 1986, 1 (2) :81-87. 被引量:1
  • 4Kiran G, Kiran H, Coban YK, et al. Fresh autologous transplantation of ovarian cortical strip to the anterior abdominal wall at the pfannenstiel incision site [ J]. Fertil Steril, 2004, 82(4) :954-956. 被引量:1
  • 5Kim SS. Fertility preservation in female cancer patients: current developments and future directions [ J ]. Fertil Steril, 2006, 85(1) :1-11. 被引量:1
  • 6Foglia RP, Laquaglia M, Dipreta J, et al. Can fetal and newborn allografts survive in an immunocompetent host [ J] ? J Pediatr Surg, 1986, 21(7) :608-612. 被引量:1
  • 7Sadeu JC, Cortvrindt R, Ron-El R, et al. Morphological and ultrastructural evaluation of cultured frozen-thawed human fetal ovarian tissue [ J ]. Fertil Steril, 2006, 85 (Suppl 1) :1130-1141. 被引量:1
  • 8Biron-Shental T, Fisch B, Van Den Hurk R, et al. Survival of frozen-thawed human ovarian fetal follicles in long-term organ culture [J]. Fertil Steril, 2004, 81(3):716-719. 被引量:1
  • 9Huhtaniemi IT, Yamamoto M, Ranta T, et al. Follicle-stimulating hormone receptors appear earlier in the primate fetal testis than in the ovary [ J ]. J Clin Endocrinol Metab, 1987, 65(6) :1210-1214. 被引量:1
  • 10Abir R, Orvieto R, Raanani H, et al. Parameters affecting successful transplantation of frozen-thawed human fetal ovaties into immunodeficient mice [J]. Fertil Steril, 2003, 80 (2) :421-428. 被引量:1

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