摘要
目的探讨丙氨酰-谷氨酰胺二肽(Ala-Gln)对大鼠心脏停搏供体肝移植缺血再灌注损伤的保护作用。方法建立大鼠心脏停搏供体肝移植模型,以供、受体是否给予谷氨酰胺分为G-d组、C-d组,G-dr组、C-dr组,检测再灌注后血清肝生化酶、肝组织还原型谷胱甘肽(GSH)及超氧化物歧化酶(SOD)水平,对肝组织进行光镜检查,并计算术后1周生存率。结果再灌注1 h,G-d组和G-dr组的血清ALT、LDH的水平均分别显著低于C-d组和C-dr组(ALT:505．53±41．77比844．13±105．91和235．59±44．89比844．83±114．72;LDH:7 059．68±1 091．28比10 989．71±1 801．23和3 454．32±563．62比11 129．70±1 920．08,P<0．05),而G-dr组血清ALT和LDH的水平又均显著低于G-d组(P<0．05)。再灌注1 h,G-d组和G-dr组的GSH的水平和SOD活性均分别明显高于C-d和C-dr组(GSH:1 204．47±153．61比847．44±59．44和1 189．86±146．82比851．74±81．74;SOD:167．24±16．10比136．17±9．68和171．59±9．87比141．57±15．79,P<0．05)。而G-dr组和G-d组的GSH水平和SOD活性差异无统计学意义(P>0．05)。G-d组和G-dr组术后1周存活率分别为55．56％(20／36)和77．78％(28／36),而C-d组和C-dr组术后1周存活率分别为25．0％(9／36)和27．78％(10／36)。结论Ala-Gln(Gln)对大鼠心脏停搏供体肝移植缺血再灌注损伤具有明显的保护作用,而这种保护作用部分与维持了供体肝脏组织中GSH的含量和提高肝脏SOD的活性有关,同时也可能与减少了肠源性内毒素与细菌易位、细胞因子及活性氧群释放至门静脉血有关。
Objective To investigate the protective role of alanyl-glutamine dipeptide pretreatment against graft warm ischemia reperfusion injury of rat liver transplantation from heart-arrested donors. Methods Estabolish OLT model from heart-arrested donors. The rats were devided into glutamine （G） and control （C） groups. Rat liver transplantation was carried out in 4 groups （ G- d, C-d, G- dr, C-dr）. liver enzymes, glutathione of reduced type （GSH） ,superoxide dismutase （SOD） of liver tissue and pathological ehanges of liver were examined. One week survival rate after operation was calculated. Results At 1 h post-reperfusion, liver enzymes of G- dand G-dr group were significantly lower than those of C-d and C-dr group [ALT： （505.53± 41.77） vs （844.13±105.91） and （235.59± 44.89） vs （844.83± 114.72）; LDH： （7059.68± 1091.28） vs （10 989.71 ± 1 801.23） and （3 454.32±563.62） vs （11 129.70± 1 920.08）,P〈0.05] ,liver enzymes of G-dr group were significantly lower than those of G-d group. The levels of GSH and SOD of G-d and G-dr groupwas obviously higher than that of C-d and C-dr group [GSH： （1204.47± 153.61） vs （847.44±59.44） and （1 189.86±146.82） vs （851.74±81.74）;SOD：（167.24± 16.10） vs （136.17±9.68） and （171.59 ± 9.87） vs （ 141.57 ± 15.79）, P 〈 0.05 ], while there was no statistical difference in the levels of GSH and SOD between G-dr group and G-d group. Hepatie pathological ehanges of G-d and G-dr group were more serve than those of C-d and C-dr group. One-week survival rate in G-d and G-dr groups was 55.56 % （20/ 36） and 77.78% （28/36）, and one-week survival rate in C-d and C-dr groups was 25.0% （9/36） and 27.78% （10/36）. Conclusions Ala-Gln （CAn） has protective effects against graft warm ischemia reperfusion injury of rat liver transplantation from heart-arrested donors. This effect was mediated at least in part by mainraining the stablity of GSH and by enhancing the activity of SOD in liver ti
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2006年第12期1473-1475,共3页
Chinese Journal of Experimental Surgery
基金
吉林省计委科技攻关项目(1997-65)
关键词
谷氨酰胺
心脏停搏
肝移植
再灌注损伤
谷胱甘肽
Glutamine
Heart arrest
Liver transplantation
Reperfnsion injury
Glutathione
