摘要
目的通过观察射频消融(RFA)治疗大鼠Walker256实体瘤后局部应用树突状细胞(DC)的疗效,探讨其临床应用的可能性。方法经Walker256细胞反复冻融获得肿瘤抗原并致敏大鼠骨髓细胞衍化的DC,大鼠腋窝皮下接种Walker256细胞获得实体瘤模型。30只SD大鼠分成三组,每组10只:对照组1仅行RFA治疗;对照组2行RFA+未致敏DC治疗;实验组行RFA+冻融抗原致敏DC治疗。超声评价治疗前后各组肿瘤体积变化,记录大鼠的荷瘤生存期,各组间比较。结果实验组瘤体平均体积在治疗后第一天与对照组无显著差异,但在治疗后第2、3天测得瘤体体积显著小于其他两组对照组(P<0.05)。实验组生存期显著长于对照组(P<0.05),对照组间瘤体体积及荷瘤生存期的差异无统计学意义(P>0.05)。结论在RFA治疗大鼠Walker256实体瘤后应用致敏DC可有效地延缓肿瘤生长速度,延长大鼠荷瘤生存期,有可能为临床治疗肿瘤提供一新途径。
Objective To assess the effectiveness of immunotherapy by dendritic cells （DCs） used after radiofrequency ablation （RFA） in rat tumor. Methods The antigen used was lysate of freeze-thraw Walker 256 cell line. The vaccine was obtained by antigen-pulsed bone-marrow derived DCs. The tumors of rats were obtained by subcutaneously transplanted Walker 256 cells into the axillary fossa of the SD rats. Thirty SD rats with tumors were randomly divided into three groups （10 per group）： control group 1 was treated by RFA only; control group 2 was treated with RFA and un-pulsed DCs; experimental group was treated with RFA and antigen-pulsed DCs. Ultrasonography was performed before and after treatment to measure the size of the tumor. The survival durations of the rats were recorded. Both of the tumor size and the survival duration were compared among different groups. Results The tumor size in experimental group was no significant difference from those of the control groups one day after treatment. However, it became significantly small from 2 days after treatment （P〈0.05）. The survival duration of the experimental group was significantly longer than those of control groups （P〈0.05）. There was no significant difference of the tumor size and survival duration between the control groups. Conclusion Using antigen-pulsed DCs after RFA can effectively decrease the growth of the tumor in the rats and prolong the survival duration of the rats. It is suggested that this may provide a potential supplement for tumor therapy.
出处
《中国医学影像技术》
CSCD
北大核心
2006年第11期1629-1631,共3页
Chinese Journal of Medical Imaging Technology
基金
广东省自然科学基金资助项目(5001696
31710)
关键词
树突状细胞
射频消融
肿瘤
大鼠
Dendritic cells
Radiofrequency ablation
Tumor
rats
