摘要
Chondus crispus（皱波角叉菜）和Mastocarpus stellatus是2种形态特征和生境极为相似的红藻，依据外部形态特征很难将它们鉴别开来。本研究测定和分析了这2种红藻的核糖体18SrRNA基因全序列，结果表明：M．stellatus的18SrRNA基因序列长度和A，T，G，C4种碱基的含量与C．crispus差异甚小，但核苷酸差异率达到了2．75％，远超过角叉菜属种内和种间的水平（0．00％～0．45％）。另外，根据该基因序列构建的系统进化树也能清楚的将C．crispus和M．stellatus区别开来，表明核糖体18SrRNA基因可从种的分类水平上对这2种红藻进行分子鉴定，具有广阔的分类学应用前景。
Chondrus crispus and Mastocarpus stellatus are two red algae with similar morphological characteristics and habitat. It is very difficult and controversial to identify them by classic taxonomic methods. In this study, the complete nuclear ribosomal 188 rRNA gene sequences of C. crispus and M. stellatus were sequenced and analyzed. The results indicated that there was little difference in the full length of 188 rRNA gene sequences and the composition of A, T, G, C bases between M. stellatus and C. crispus, but the nucleotide variation rate for 18S rRNA gene （2.75 % ） in M. stellatus was far larger than that in intraspecies and interspecies of Chondrus Stackhouse （0.00 % -0.45 % ）. Besides, the phylogenetic trees inferred from the sequences could discriminate the two red algal species clearly. These results indicate that nuclear ribosomal 18S rRNA gene sequences could identify C. crispus and M. stellatus as a molecular tool at the species level and may be widely used in red algal taxonomy.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第6期946-952,共7页
Periodical of Ocean University of China
基金
中国科学院农业扶贫专项项目
中国科学院海洋研究所实验海洋生物学重点实验室开放课题资助
