摘要
目的：探讨组胺受体１、组胺受体２（Ｈ１Ｒ，Ｈ２Ｒ）在哮喘致病中的作用。方法：用放射配基结合分析法测定了１２只哮喘豚鼠及１２只正常豚鼠外周血淋巴细胞（ＰＢＬ）和肺组织的Ｈ１Ｒ和Ｈ２Ｒ，并对哮喘豚鼠ＰＢＬ的Ｈ１Ｒ和Ｈ２Ｒ与肺组织的Ｈ１Ｒ和Ｈ２Ｒ密度作了相关分析，实验所用配基分别为Ｈ１Ｒ和Ｈ２Ｒ选择性阻断剂￣３Ｈ－ｍｅｐｙｒａｍｉｎｅ和￣３Ｈ－ｔｉｏｔｉｄｉｎｅ。结果：哮喘豚鼠ＰＢＬ和肺组织Ｈ１Ｒ密度均升高，而Ｈ２Ｒ密度均下降（Ｐ＜０．０５，Ｐ＜０．０１），哮喘豚鼠ＰＢＬ的Ｈ１Ｒ，Ｈ２Ｒ变化分别与肺组织Ｈ１Ｒ，Ｈ２Ｒ呈正相关（Ｐ＜０．０５，Ｐ＜０．０１）。结论：Ｈ１Ｒ和Ｈ２Ｒ失调与哮喘的致病有关，外周血淋巴细胞Ｈ１Ｒ和Ｈ２Ｒ的变化在一定程度上可以反映肺组织Ｈ２Ｒ和Ｈ２Ｒ的变化。
Objective:To make sure whether the disorder of H1R and H2R is involved in the pathogenesis of asthma. Mehtods : The [3H]-mepyramine and [3H]-tiotidine,which are H1 receptor (H,R) and H2 receptor (H2R) selective antagonists respectively,were selected in this study to detect the H1R and H2R of peripheral blood lymptiocytes (PBL) and lung homogenate in 12 guinea pigs with asthma or in 12 normal guinea pigs. The correlation of PBL H1R, H2R and lung homogenate H1R, H2R were also studied. Results : The H1R density of PBL and lung homogenate increased and the H2R density of PBL and lung homogenate decreased. Both H1R and H2R densities of PBL positively correlated with those of lung homogenate. Conclusion :The disorder of H1R and H2R is involved in the pathogenesis of bronchial asthma and the changes of PBL H1R and H2R may somehow represent those of lung H1R and H2R.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1996年第5期474-476,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
