摘要
通过应用抗人T淋巴细胞及其亚群单克隆抗体OKT系列,以间接免疫萤光法检测80例重型颅脑损伤患者外周血T淋巴细胞及其亚群,以酵母多糖法测定红细胞免疫功能,同时以单相放射状免疫扩散法检测血清免疫球蛋白及补体C_3浓度。结果发现:OKT_3^+、OKT_4^+百分率下降,OKT_8^+百分率上升,OKT_4/OKT_8^+比值倒置;红细胞C_(3b)受体花环(RC_(3b)RR)和红细胞免疫促进百分率(RFER)下降,红细胞免疫复合物花环(RICR)和红细胞免疫抑制百分率(RFIR)上升;血清IgG、IgM、IgA和补体C_3下降;与正常对照组比较差异显著(P<0.O1)。结果表明:重型颅脑损伤患者免疫功能处于抑制状态。其原因可能与中枢神经系统功能障碍和内分泌紊乱有关。免疫功能的动态观察对判断重型颅脑损伤患者预后有重要意义。
Peripheral blood lymphocytes and their subpopulations were examined by using OKT series of monoclonal antibody and indi- rect immunofluorescence technique in 80 pa- tients with severe craniocerebral injury. Ery- throcyte immune function was detected with saccharomycete. Serum immunoglobulin and complement C3 was investigated with singular radioimmunodiffusion. The results demon- strated that; the percentage of OKT3^+, OKT4^+ decreased and OKT8^+ increased; ery- throcyte C3b receptor rose and the enhancing ratio of erythrocyte immunity decreased; ery- throcyte immune complex rose and the inhibit- ing ratio of erythrocyte immunity increased. Serumal IgG, IgM, IgA, and Complement C3 decreased. There was a significant difference between the normal control and experimental group. The results showed that the immune function in patients with severe craniocerebral injury was in the state of inhibition. This was in close relationship with impairment of the function of the central nerve system and en- docrine system. The measurement of the im- mune function is a valuable indicator of the prognosis of patients with severe craniocere- bral injury.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1996年第6期344-345,共2页
Chinese Journal of Experimental Surgery
关键词
颅脑损伤
淋巴细胞
亚群
红细胞
免疫球蛋白
craniocerebral injury
Tlymphocytic subpopulation
immunoglobin erythrocyte immunity
