摘要
目的应用差异蛋白质组学技术,研究深低温和常温停循环后大鼠海马组织蛋白质的变化。方法采用深低温停循环模型,取大鼠海马组织,通过双向电泳、分离蛋白,然后通过胶内酶切、生物质潜分析差异的蛋白质点,鉴定出变化的蛋白质。结果通过Image Master软件分析报告发现有差异的Ratio值大于1．5的蛋白考染点14个。通过对这些蛋白考染点进行质谱鉴定,鉴定出28个蛋白质,其中4个为同一种蛋白,实际的蛋白数为24个。它们是细胞骨架蛋白、介导代谢的酶类、参与核酸合成、参与氧化直激反应的蛋白质及未知蛋白。结论鉴定出的差异蛋白质可能与深低温脑保护作用有关,某些蛋白质在低温脑保护中的作用尚未报道。
Objective To evaluate the effects of deep hypothermia on protein expression in hippocampus following circulatory arrest by proteomics technique. Methods The hippocampus was taken following 5 minutes circulation arrest with deep hypothermia or normothermia. The total proteins mixture extracted in protein lysate was separated with two dimensional gel electrophoresis. The differential protein spots were identified by matrix-assisted laser desorption-time of flight mass spectrometry. Results Fourteen protein spots were found with statistically significant change, its ratio value was 〉 1.5. Twenty eight products of protein spots were identified by mass spectroscopy, four of them were repeated, the actual products of proteins were 24. They included cytoskeletons, energy-related enzymes, nucleic acids synthetic proteins, oxidative and stress proteins, as well as unknown proteins. Conclusion Different expressions of hippocampus following circulatory arrest with deep hypothmia or normothermia may be related to the protective mechanisms of deep hypothermia.
出处
《中华神经外科杂志》
CSCD
北大核心
2006年第11期651-654,共4页
Chinese Journal of Neurosurgery
基金
国家重点基础研究项目(2005CB522604)
上海市科委重大项目基金(04DZ14005)
上海市医学领军人才基金资助
关键词
深低温
蛋白质组
电泳
凝胶
双相
生物质谱
大鼠
Deep hypothermias
Proteomics
Electrophoresis, gel, two-dimensionsl
Mass spectrometry
Rats
