摘要
以纯化的新城疫病毒（Newcastle disease virus，NDV）弱毒株La Sota为免疫原，接种6周龄～8周龄Balb／c小鼠。最后一次加强免疫后3d，取小鼠脾细胞与骨髓瘤细胞（SP2／0）融合。以纯化的NDV建立了筛选单抗的间接ELISA，经多次检测，3次亚克隆，共获得5株NDV特异性单抗，这5株单抗与NDV不同分离株的反应性存在差异，表明部分NDV毒株抗原性发生了变异。
The Balb/c mice were immunized hy the purified Newcastle disease virus （NDV） LaSota strain. The spleen cells of the mice were hybridized with SP2/0 three days after the last immunization, The indirect ELISA was developed by using the purified NDV as antigen and used to screen monoclonal antibodies （MAbs）. As a result, five MAhs with specificity to the NDV were obtained. The reacting patterns of MAbs with different NDV strains indicated that the antigenicity happened in a part of NDV isolates.
出处
《动物医学进展》
CSCD
2006年第11期65-68,共4页
Progress In Veterinary Medicine
基金
山东省十五科技攻关项目(022020103)
关键词
新城疫病毒
单抗
抗原性
Newcastle disease virus
monoclonal antibody
antigenicity
