摘要
目的寻找一种简便、快速、有效的检测高产AmpC酶的方法。方法用纸片扩散法对1 935株大肠埃希菌和克雷伯菌属细菌进行AmpC酶的初筛试验,然后用EDTA纸片法、头孢西丁三维试验和多重聚合酶链反应(PCR)同时对327株初筛阳性细菌进行AmpC酶的检测,并进行方法学比较。结果PCR、头孢西丁三维试验、EDTA纸片法阳性菌株分别为54、85、94株,阳性率分别为2.79%、4.39%、4.86%,EDTA法与三维试验的符合率为97.25%,2种检测方法差异无显著性(P>0.05);EDTA对质粒AmpC酶的检测率高于三维试验。结论EDTA纸片法用于这2种临床菌株中持续高产AmpC酶的检测,方法简便,结果可靠,无需特殊仪器支持,可在临床实验室推广使用。
Objective To detect the hyperproduced AmpC β-lactamases in Escherichia coli and Klebsiella spp. Methods 1 935 strains of Eseherichia coli and Klebsiella spp were screened by CLSI standard disk diffusion. EDTA disk, 3-D test and muhiplex polymerase chain reaction （PCR） were performed on 327 strains. Results 54 （2.79%）, 85 （4.39%） and 94（4.86% ） were positive in EDTA disk test, 3-D test and Multiplex PCR respectively. Coincidence between EDTA disk test and 3-D test was 97.25% （P 〉0.05）. Condusions EDTA disk test is a simple, rapid and effective method to detect the hyperproduced AmpC β-lactamases in Escherichia coli and Klebsiella spp.
出处
《检验医学》
CAS
北大核心
2006年第6期625-628,共4页
Laboratory Medicine
