摘要
采用ITS-nrDNA-RELP、测序技术、RAPD(随机扩增多态性DNA)分子标记技术,对我国松杨栅锈菌不同地域的5个生理小种11个菌系进行了遗传多样性分化研究.结果表明,该菌在我国的遗传分化与地理来源相关,可分为西部地理群和北方地理群.西部地理群又可分为高山森林生态型(HMF)和平原生态型(WPL).小种遗传分化不一定与致病性分化一致.t检验表明,各生理小种RAPD遗传多样性指数无明显差异,高山森林小种遗传多样性指数(0·5172)略高于平原小种遗传多样性指数(0·5089).核糖体基因转录间隔区高度保守,不适合该菌种内群体遗传多样性分化研究.
By using ITS- nrDNA-RELP, ITS sequencing, and RAPD molecular marker, this paper studied the genetic diversity of 11 isolates in 5 races of Melampsora larici-populina from different regions. The results indicated that the genetic differentiation of M. larici-populina was correlated to its geographical distribution, which could be grouped into northern and western geographic populations, and the latter could be grouped into high-mountain forest ecologic type （HMF） and western plain ecologic type （WPL）. The genetic differentiation of the race was not always corresponded with the pathogenic one. T test manifested that the genetic diversity of the 5 races had no statistic difference in RAPD markers, and the diversity index of HMF was 0. 5172, being slightly higher than that of WPL （0. 5089）, The ITS sequence of ribosome DNA was strictly conservative, and not suitable for the genetic diversity study of M. larici-populina intra-species populations.
出处
《应用生态学报》
CAS
CSCD
北大核心
2006年第11期2102-2106,共5页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(30471394)
教育部博士点基金项目(2004712001)
陕西省自然科学基金资助项目(2004C113).
关键词
松杨栅锈菌
分子标记
遗传多样性指数
Melampsora larici-populina, Molecular marker, Genetic diversity index
