摘要
番茄红素是一种重要的类胡萝卜素,具有许多生物功能和生物活性,尤其在保护人类健康方面起着重要的作用。随着番茄红素生物合成途径的阐明及其相关基因的克隆,运用基因工程手段调控番茄红素的合成已经成为可能。首先综述了番茄红素生物合成途径及合成途径中相关基因的克隆,然后对近年来构建的番茄红素基因工程菌进行了全面的总结,包括:运用DNA重组技术使异源微生物生产番茄红素;通过表达特定基因从而提高霉菌等产番茄红素的量;分析了提高番茄红素产量和构建新的基因工程菌中存在的主要问题,并展望了未来的研究方向。
Lycopene-a kind of important active compound of caroteinoids, is greatly beneficial to human health with its diverse biological functions. With the elucidation of lycopene biosynthetic pathway and cloning genes of relative enzymes from microorganisms, it is possible to regulate lycopene biosynthesis via genetic engineering. The biosynthesis pathways of lycopene and gene cloning of lycopene biosynthetic enzymes in microorganisms were reviewed, and gene engineering strains documented in previous works including： E. coli and yeast constructed by genetic recombination, mold strains enhanced the ability of producing lycopene by gene manipulation were summarized. At last, compared with the present methods, the problems existed in the process of construction were pointed out.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2006年第11期81-86,共6页
China Biotechnology
基金
国家自然科学基金资助项目(20376007)
关键词
番茄红素
合成途径
代谢酶基因
基因工程菌
Lycopene biosynthesis pathway Gene of enzyme Gene engineering strain
