摘要
根据已报道的哺乳动物朊蛋白基因序列设计了1对引物,采用PCR方法扩增了16只非洲狮的朊蛋白基因,序列分析结果表明,所得到的非洲狮朊蛋白基因片段长678 bp、编码226个氨基酸的前体蛋白,其核苷酸序列的同源性为99.79%以上,发现了4个核苷酸多态性位点,无氨基酸变异。经与已报道的猫、貂、绵羊、牛、鼠和犬等哺乳动物的氨基酸序列进行比较,与猫(AF003087,96.7%)和绵羊(96.2%)的氨基酸同源性最高。
A pair of primers was designed according to reported prion protein genes（PRNP） in mammalian animals. The PRNPs of 678 bp encoding 226 amino acids were amplified in 16 African lions by PCR, and the homology among the PRNPs from the 16 lions was more 99. 79%. Four single nucleotide polymorphisms were found, but no variation in amino acids. Compared cat, mink, sheep, cattle, mouse and dog, African lion shared the highest homology with cat（AF003087） and sheep in amino acid.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2006年第11期864-867,共4页
Chinese Veterinary Science
基金
国家自然科学基金项目(3040032530500371)
关键词
朊蛋白基因
非洲狮
多态性
克隆
序列分析
prion protein gene
African lion
polymorphism
cloning
sequence analysis
