摘要
以何首乌幼嫩茎段为外植体,采用二次回归正交设计方法研究了何首乌组织培养快繁和继代培养基中6-BA和NAA浓度配比关系。通过对所建模型的分析得出:6-BA的最优浓度为1.26￣1.99mg/L,NAA的最优浓度为0.05￣0.48mg/L,其可信度达95%。
Quadratic regressive factorial experiment was used to optimize concentration of 6-BA and NAA in MS medium for shoot propagation from the stem segments of Polygonum multiflorum Thunb. The result showed that the most of shoot achieved by the 6-BA and NAA concentrations were in a rang of 1.26- 1.99mg/L and 0.05-0.48mg/L respectively.The probability of the event was 95%.
出处
《中国农学通报》
CSCD
2006年第11期173-175,共3页
Chinese Agricultural Science Bulletin
基金
重庆市自然科学基金资助项目"根茎类中药材退化原因及其共性控制技术研究"(8024)
关键词
二次回归正交设计
何首乌
组织培养
6-BA
NAA
Quadratic regression factorial experiment, Polygonum multiflorum Thunb, Tissue culrure, 6-BA, NAA
