摘要
采用不同种类和浓度的生长素诱导葡萄组培苗瓶外生根,并观察组培苗在不同处理基质、不同的温度、不同的生理年龄条件下生根的效果。通过优化技术参数,建立了葡萄组培苗瓶接生根的技术优化体系,结果表明,用浓度为0.3mg/L的IBA浸蘸处理的插条,在温度为27￣29℃的环境条件下在基质珍珠岩上培养,瓶苗茎段的生根率可达85.5%,移栽后苗木的成活率达98%,相对于其他方法有了较大的提高。该技术的建立有助于实现葡萄工厂化育苗技术的商业化扩大。
The paper researches the different type and the density growth hormone which mduct rooting tissue culture the seedling in grape outside the bottle, and, the effect of tissue culture the seedling＇s rooting in grape was observed in the different processing matrix, the different temperature, and the different physiological age condition .we established the grape group to cultivate the technical optimization system which can directly took root outside the bottle. The result indicated that use 300mg/L IBA soaks with the density dips processing the cutting, 27 -29 ℃ environmental condition raises under the matrix pearlite, the root rates to be possible to reach 85.5 %, after transplanted nursery stock＇s survival rate to reach 98 %, was opposite had a bigger enhancement to the alternative means, establishment of technology is helpful to the realization of commercial expansion.in grape factorization culture of seedling.
出处
《中国农学通报》
CSCD
2006年第11期75-78,共4页
Chinese Agricultural Science Bulletin
基金
新疆省农业厅资助项目"设施果树栽培品种的引进与繁殖"(2001EA890001)
关键词
葡萄组培苗
瓶外生根
生长素
Tissue culture the seedling of grape, Rooting outside the bottle, Growth hormone
