摘要
目的分离、纯化具有降血糖作用的仙人掌多糖组分。方法经热水提取、乙醇沉淀、DEAE Sepharose fast flow离子交换色谱和Sephadex G系列凝胶滤过色谱纯化得到5种仙人掌多糖组分。醋酸纤维薄膜电泳检测多糖纯度,凝胶色谱测定相对分子质量,高效液相色谱测定糖的组成。结果5种多糖都已达到电泳纯。它们的相对分子质量依次为2.0×103、1.0×106、4.0×103、9.2×105、5.0×103。ODP1由鼠李糖组成,ODP2可能由鼠李糖和葡萄糖组成,ODP4可能由鼠李糖和D-半乳糖组成。ODP3和ODP5分别由鼠李糖和一未知糖组分组成。结论仙人掌多糖的提取没有必要采用脱蛋白、脱色素步骤,本实验所提取的仙人掌多糖是均一的。
Objective To isolate, purify, and identify the Opuntia dillenii polysaccharide (ODP). Methods Fresh cactus was extracted by hot water, precipitated by ethanol, fractionated by DEAE Sepharose fast flow, separated by Sephadex G series, and treated by freeze-drying. After the above purification, five polysaccharides were obtained. Purity of them was examined by electrophoresis, relative molecular weight of them was measured by gel chromatography, and chemical composition was determined by HPLC. Results All of ODP were proved to be homogenous, relative molecular weight of them were 2.0×103, 1.0×106, 4.0×103, 9.2×105, and 5.0×103. ODP1 was composed of rhamnose only, ODP2 was made up of rhamnose and D-glucose, ODP4 contained rhamnose and D-galactose, ODP3 and ODP5 consisted of rhamnose and another unidentified monosaccharide, respectively. Conclusion The deproteinized or decolorized procedure is not necessary for the purification of ODP, and all of ODP are identified to be homogenous.
出处
《中草药》
CAS
CSCD
北大核心
2006年第11期1641-1645,共5页
Chinese Traditional and Herbal Drugs
基金
广东省高校自然科学基金资助项目(Z03062)
湛江市科技招标计划项目(湛财企[2004]98号)
