摘要
本研究结果显示:血吸虫成虫细胞在体外培养中呈半悬浮聚集生长状态,4代内的培养细胞及冻存复苏细胞存活率达90%,并在各代培养中均可见细胞分裂相;传5代细胞的染色体核型为8对16条。超微结构既观察到形态正常细胞,也可见形态异常细胞。表明1640-40特定培养基对血吸虫成虫中的部分细胞体外传代培养获得成功。
Cultivation of cells from 30-day old Schistosoma japonicum （S.j） adult worms showed that the growth features of the cells were semi-floating and accumulative. The survival rate of the primary cells, passage cells prior to the 5th generation and recovered cells was all up to 90%. Phases of cell division were observed during cultivation. Chromosome karyotype of the 5th generation cells possessed diploid feature of the blood-flukes （2n=8 in number）. Uhrastructure of the 5th generation cells showed that four types of cells in normal morphology and three types of cells in abnormal morphology were both viewed. It is suggested that some of the cells from S.j adult worms were subcuhured successfully in the 1640-40 defined medium.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2006年第5期395-397,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家自然科学基金(No.30170840和No.30570952)~~
关键词
日本血吸虫
成虫
细胞
传代培养
超微结构
染色体核型
Schistosoma japonicum
Adult worm
Cell
Passage culture
Ultrastructure
Chromosome karyotype
