摘要
目的探讨人p27kip1基因转染与化疗药联合应用对舌癌细胞生长的影响。方法从正常组织中克隆人p27kip1基因连接至pcDNA3真核表达载体上,应用脂质体将重组质粒转染舌癌Tca8113细胞,观察外源目的基因在靶细胞中的整合及表达,以及转染与未转染细胞加用化疗药物后的生长情况。结果成功克隆p27kip1cDNA并构建p27kip1真核表达载体,将其导入Tca8113细胞中,经G418筛选得到稳定表达p27kip1基因的细胞株,转染p27kip1基因的细胞显示了对化疗药长春新碱的敏感性增高。结论p27kip1基因转染提高了化疗药对舌癌Tca8113细胞生长抑制作用。
Objective To investigate the effect of human p27^kip1 gene transfection with chemotherapeutics on the growth of human tongue cancer cells. Methods Clone human p27^kip1 gene from normal tissue had been connected to eukaryotic expression vector pcDNA3, then it was transferred the recombinant plasmid with liposome to Tca8113 cell of tongue cancer. The integration and expression of exogenous objective gene in the target cell and the growth of transferred cell and non - transferred cell was observed. Results We cloned p27^kip1 cDNA successfully and constructed p27^kip1 eukaryotic expression vector, then we put it into Tca8113 cell and obtained the cell strain that expressed p27^kip1 gene stable after screening with G418. The result was the cell transferred with p27^kip1 gene shown an increase in sensibility to vincristinum sulfate. Conclusion p27^kip1 gene transfection increased the inhibit role of chemotherapeutics in the growth of Tca8113 cell.
出处
《现代口腔医学杂志》
CAS
CSCD
北大核心
2006年第6期594-597,共4页
Journal of Modern Stomatology
关键词
P27^KIP1
克隆
癌
鳞状细胞
转染
化疗药物
p27^kip1 Clone Carcinoma,squmaous cell Transfection Chemotherapeautic medicine
