摘要
采用细胞培养，￣３Ｈ－ＴｄＲ同位素示踪及ＭＴＴ显色技术观察了氯化镓（ＧａｌｌｉｕｍＣｈｌｏｒｉｄｅ，ＧＣ）对人胃低分化粘液腺癌ＭＧｃ_（８０－３）细胞株的生长抑制作用及ＤＮＡ合成的影响，并以铁为实验因子探讨镓抑瘤作用机制。结果表明：稼能使ＭＧｃ_（８０－３）人胃癌细胞￣３ＨＴｄＲ掺入显著减少，抑制癌细胞ＤＮＡ合成。镓不仅使癌细胞生长抑制，还可使癌细胞死亡。这种抑制作用可被Ｆｅ￣（＋３）的加入而减弱。提示：ＧＣ抑制癌细胞生长的机制可能与癌细胞铁利用障碍致ＤＮＡ合成低下有关。
This study, using gastric carcinoma cell culture, ￣3H-TdR isotope incorporation and MTT assay technique, observed the growth inhibition effect of gallium chloride(GC)on poorly differentiated mucoid adenocarcinoma of human stomach MG_(c80-3) cell line and the effect of GC on DNA synthesis. Using FeCl3 as an objection, we investigated the mechanism of the growth inhibition effect of gallium on MG_(c80-3) cell.The result suggested that ￣3H-TdR isotope uptake was markedly decreased by GC,and GC could inhibit DNA synthesis.GA_(+3) can not only make cancer cell grow inhibitively,but also make the cell die.This inhibitive effect can be decreasedby Fe_(+3).The result indicates that the mechanism of GC inhibiting cancer cell may be related with the block of uitilizing Fe in cell, which results in decrease of DNA synthesis.
出处
《西安医科大学学报》
CSCD
1996年第2期175-178,共4页
Journal of Xi'an Medical University(Chinese)
关键词
镓盐
胃癌细胞
DNA合成
抑瘤机制
gallium salt
gastric carcinoma cell
DNA synthesis
antitumor mechanism
