摘要
目的:研究冠心病患者巨噬细胞肝X受体(LXR)及其下游的一些目的基因表达和胆固醇外流特点。方法:分离冠心病患者组和对照组外周血的单核细胞,并转化为巨噬细胞。在TO-901317的刺激下,观察巨噬细胞的aopA-I介导胆固醇外流的变化和LXR以及其下游一些目的基因的mRNA表达。结果:冠心病患者的巨噬细胞,其一些影响胆固醇代谢及炎症反应的基因表达受到了影响,aopA-I介导的胆固醇外流能力下调,对刺激LXR后的反应性也降低。结论:冠心病患者的巨噬细胞胆固醇外流功能下降,其一些影响胆固醇代谢及炎症反应的基因表达发生了改变,对刺激LXR信号的反应性下降。提示巨噬细胞的LXR信号途径可能是冠心病发病机制中的重要环节,也是冠心病治疗潜在靶点。
AIM ： To study the characteristic of liver X receptor alpha （LXRct） , it＇s target gene expression and cholesterol efflux from human macrophages in coronary atherosclerotic disease （CAD） patients. METHODS： Human monocyte - derived macrophages from CAD patients and controls were collected. Before being detected apoA - I - mediated human monocyte - derived macrophage cholesterol efflux and LXRα and mRNA expression of its target gene, the macrophages were induced with or without TO -901317. RESULTS： Compared with control normal macrophage, the mRNA levels of LXRα and its target gene expression were changed, and the macrophage cholesterol efflux was decreased in CAD patients. After stimulationwith TO -901317, the reactive capacity of LXR was also decreased from human monocyte - derived macrophage of CAD patients. CONCLUSION： The changes of cholesterol eftlux and some gene expression in macrophages may be the pathogenetic cause atherosclerosis, and macrophage LXR activity may offer potential therapeutic benefit in the treatment of CAD.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第11期2146-2150,共5页
Chinese Journal of Pathophysiology
