摘要
目的探讨移植肾组织及血清中透明质酸(HA)、层粘连蛋白(LN)及Ⅳ型胶原(C_(-Ⅳ))含量与急性排斥反应的关系。方法以雄性SD大鼠为供者,Wistar大鼠为受者,采用改进的Blom法制作大鼠原位左肾移植模型,设同种移植对照组、同种移植用药组(术后腹腔注射环孢素A 10 mg·kg^(-1)·d^(-1)),另设同系移植组(供、受者均为Wistar大鼠)和假手术组(Wistar大鼠仅切除右肾,不行移植),每组20只大鼠。每组分别于术后3、5、7及9 d各随机选取5只大鼠,从下腔静脉采血2 ml,随后切取移植肾(假手术组切取左侧肾脏),用放射免疫分析法测定肾组织匀浆及血清中HA、LN及C_(-Ⅳ)的含量,并行肾组织切片,根据“Banff 97”标准进行病理诊断。结果从术后5d开始,同种移植对照组的肾组织匀浆及血清中HA、LN及C_(-Ⅳ)的浓度均显著高于同系移植组、假手术组及同种移植用药组(P＜0．01)；血清中HA、LN及C_(-Ⅳ)的浓度与肾组织匀浆中HA、LN及C_(-Ⅳ)的浓度呈正相关(r分别为0．960、0．934和0．847,P值均为0．000)。病理检查证实,同种移植对照组共有17个肾脏发生急性排斥反应,同种移植用药组共有4个肾脏发生急性排斥反应,它们的Banff急性排斥反应指数与移植肾组织匀浆中及血清中HA、LN及C_(-Ⅳ)的浓度均呈正相关(P＜0．01)。结论HA、LN及C_(-Ⅳ)在肾移植急性排斥反应的发生及发展中起重要作用,肾移植术后监测血清中HA、LN及C_(-Ⅳ)的浓度有助于急性排斥反应的诊断。
Objective To study the change of the hyaluronic acid （HA）, laminin （LN） and collagen type Ⅳ （C-Ⅳ） within the renal grafts and in serum during acute rejection and to investigate their relationship. Methods Male Wistar and Sprague-Dawley rats were used as donors and recipients, respectively. Rat orthotopic kidney transplantation was performed according to a modification of the method decribed by Blorn. Experimental rats were randomly divided into 4 groups： homotransplantation rats treated with cyclosporine, isotransplantation, pseudooperation and controls. Animals were subsequently killed at defined time points for determination of the extracellular matrix （ECM） parameters （HA, LN and C-Ⅳ ） within the graft and in serum by radioimmunoassay. Results Significant increases in HA, LN and C-Ⅳ levels within the renal grafts were found in the rejection group as compared with the non-rejection groups. Serum levels of HA, LN and C-Ⅳ were also significantly elevated in the rejection group at diagnosis of rejection. Serum HA, LN and C-Ⅳ levels were correlated with those within the renal grafts. Histologic examination revealed that 4 cases developed acute reiection in homotransplantation rats treaded with cyclosporine, 17 cases in controls. HA, LN and C-Ⅳ levels within the renal grafts were correlated with acute rejection Banff scores. There was correlation between serum levels of HA, LN and C-Ⅳ and acute rejection Banff scores （P〈0. 01 ）. Conclusion HA, LN and C-Ⅳ play an important role in the pathogenesis of acut allograft rejection. In addition, serum levels of HA, LN and C-Ⅳ may be a sensitive marker of acute rejection in the postoperative period of renal transplantation.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2006年第11期652-655,共4页
Chinese Journal of Organ Transplantation
关键词
透明质酸
层粘连蛋白
胶原Ⅳ型
肾移植
移植物排斥
Hyaluronic acid
Laminin
Collagen type IV
Kidney transplantation
Graft rejection
