摘要
目的:建立高血压伴胰岛素抵抗动物模型并观察血清脂联素、肿瘤坏死因子α的变化。方法:实验于2005-07/09在海军总医院中心实验室完成。选择6周龄雄性Wistar大鼠20只,随机数字表法分为两组,对照组(n=10)以普通标准饲料+普通自来水喂养i果糖组(n=10)以普通标准饲料+10%果糖水喂养,连续喂养14周,于喂养后第8和14周末,采用尾部测量法测定大鼠血压,胰岛素抵抗指标采用胰岛素敏感性指数=-ln[空腹血糖×空腹胰岛素],采用放射免疫法测定血清脂联素和肿瘤坏死因子α的浓度。结果:纳入动物20只,均进入结果分析。①第8周末果糖组肿瘤坏死因子α水平与对照组相比明显升高[分别为(2.21±0.34),(1.84±0.25)μg/L,P<0.05],胰岛素敏感性指数、脂联素明显下降[分别为-4.64±0.12,-4.23±0.27;(4.76±0.67),(5.83±0.99)mg/L,P<0.01]。②第14周末两组间胰岛素敏感性指数、脂联素和肿瘤坏死因子α的差异更加明显,第14周末果糖组胰岛素敏感性指数、脂联素与第8周末相比明显降低[-5.05±0.32,-4.64±0.12;(3.60±0.50),(4.76±0.67)mg/L,P<0.01],肿瘤坏死因子α明显升高[分别为(2.53±0.39),(2.21±0.34)μg/L,P<0.01]。③相关性分析表明,脂联素与空腹胰岛素、胰岛素敏感性指数和肿瘤坏死因子α水平的相关性显著(r=-0.564,-0.641,-0.538,P<0.05)。结论:10%果糖水喂养的Wistar大鼠,具有典型的高血压伴胰岛素抵抗的特点;胰岛素敏感性降低的同时伴有血清脂联素下降和肿瘤坏死因子α的升高。
ATM： To establish rat models of hypertension and insulin resistance, and study the changes of serum adiponectin and tumor necrosis factor （TNF） α.
METHODS： The experiment was conducted in the Central Laboratory of Naval General Hospital from July to September 2005. Twenty Wistar male rats of six-week-old were selected and divided into two groups： control group （n=10） and fructose group （n=10）. Rats in the control group were fed with standard diet and normal water and rats in the fructose group were fed with standard diet and 10% of fructose water. Rats in both groups were fed for 14 continuous weeks, and the blood pressure was detected at 8 and 14 weeks after feeding with tail-cuff method. The insulin sensitivity index （ISI） was taken as the insulin resistance index [ISl=-In （fasting blood glucose xfasting insulin）]. The level of serum adiponectin and TNFα were determined by radioimmunoassay
RESULTS： A total of 20 enrolled rats were involved in the analysis opf resuhs.（1）The level of TNFα at the 8^th week was obviously increased in the fructose group than the control group [respectively （2.21±0.34）, （1.84±0.25）μg/L,P 〈 0.05], while the ISI and serum adiponectin in fructose group were significant decreased [-4.64±0.12,-4.23±0.27;（4.76±0.67）, （5.83±0.99） mg/L,P 〈 0.01]. （2）The differences in ISI, adiponeetin and TNF α between two groups at the 14^th week were more significant. The ISI and level of adiponeetin in the fructose group was significantly decreased at the 14^th week than the 8^th week [-5.05±32,-4.64±0.12; （3.60±0.50）, （4.76±0.67） mg/L,P 〈 0.01], while the level of TNFα was significantly increased [respectively were （2.53±0.39）, （2.21±0.34）μg/L,P 〈 0.01]. （3）Correlation analysis showed that adiponeetin was significantly related to fasting insulin, ISI and level of TNF α（r=-0.564,-0.641 ,-0.538,P 〈 0.05 ）.
CONCLUSION： Rats fed with 10% of fructose water are characteristic of typical hypertensio
出处
《中国临床康复》
CSCD
北大核心
2006年第44期60-62,共3页
Chinese Journal of Clinical Rehabilitation
