摘要
将人工采集的长白猪精液分为六组,其中一组为空白对照组,其余五组为实验组,分别加入5、10、25、50和100ns/ml的血小板活化因子(plarelet—activating factlr,PAF),于37℃恒温水浴锅中温育5,15,25,35,45,60min。显微镜下检测精子活率,同时作精子涂片,经固定染色后测算顶体反应率(Acrosomcreactloll,AR)。结果表明,(1)、PAF浓度为10ng/ml的实验组与对照组相比,在温育15—60rain期间,精子活率都极显著提高(P<0．01)；(2)、PAF浓度为5ng/ml的实验组与对照组相比,在温育25～45min期间,精子活率都极显著提高(P＜0．01)；(3)PAF浓度为5、10、25和50ng/ml组的精子在温育60min内顶体反应率均极显著高于对照组(P＜0．01)；(4)浓度为100ng/ml的PAF组与对照组相比,顶体反应率差异不显著(P＞0．05),表明一定浓度的PAF可提高长白猪精子的活率及顶体反应率。
Divide the artificially gathered seminal fluid of a long white pig into six groups . One of these groups is left to be compared with,and the other five are used for experimenting. AddS, 10,25,50, and 100ng/ml platelet - activating factor （PAF） into the five groups and breed them in 37℃ thermostatical water for 5,15,25,35,45, 60minutes. Measure fixing dyes. The result shows： （ 1 ） Compared with the contral group, the group of 10ng/ml PAF,bred for 15 -60 minutes ,the sperm living rate
出处
《山东农业大学学报（自然科学版）》
CSCD
北大核心
2006年第3期389-391,396,共4页
Journal of Shandong Agricultural University：Natural Science Edition
