摘要
目的:观察姜黄素对白血病细胞株K562细胞拓扑异构酶TopoI表达的影响。方法:流式细胞术及RT-PCR法分析姜黄素作用K562细胞后TopoⅠ蛋白及mRNA含量的变化。结果:TopoI在K562细胞中高表达,TopoI蛋白的平均荧光强度为84.31±4.26,显著高于阴性对照组3.82±0.17(P<0.01);流式细胞术及RT-PCR法均提示姜黄素能显著抑制TopoⅠ的表达,25μmol/L姜黄素能抑制K562细胞TopoI蛋白的表达超过一半以上,并且随着剂量的增加,姜黄素的抑制作用逐步增强。结论:姜黄素可通过抑制TopoI的表达来抑制K562细胞的增殖,是一种新的TopoI抑制剂,具有良好的临床应用前景。
Objective,To investigate the effect of curcumin on topoismerase I of leukemia K562 cells. Method： FCM and RT-PCR were used to analyze the change of Topoi mRNA and protein. Result; Topoi was highly expressed in K562 cells. Mean fluorescence intensity of Topoi protein in blank group （84.31±4.26） was significant- ly higher than that in control group （3.82±0. 17, P 〈0. 001）. Curcumin can significantly inhibit the expression of Topo I by FCM and RT-PCR analysis. 25 μmol/L curcumin can inhibit the expression of Topoi. The inhibitory effect of curcumin increased with increasing dosage of curcumin. Conclusion;Curcumin can inhibit the proliferation of K562 cell through inhibiting the expression of Topoi. Curcumin is a new Topoi inhibitor and has a good clinical application prospect.
出处
《临床血液学杂志》
CAS
2006年第6期352-354,共3页
Journal of Clinical Hematology
基金
国家自然科学基金资助项目(No:30472267)
