摘要
目的探讨富含α-亚麻酸(α-LNA)的胡麻籽油(亚麻油)对子代小鼠脑发育及功能的影响。方法取性成熟小鼠45只,雌雄为2∶1,将怀孕雌鼠随机分为4组,分别给予胡麻籽油2g(A组)、5g(B组)、10g(C组)/kg体重和对照组(0组),连续给孕鼠经口灌胃至仔鼠断乳,母鼠处死,将仔鼠雌雄分笼,雄鼠立即处死进行脑体重量测定并计算脑体比;再将雌鼠C组分为C组和D组,A、B、C组雌鼠继续按母鼠剂量补充胡麻籽油,每天1次,D组不补充胡麻油继续喂养;5周后进行小鼠避暗和水迷宫实验,测定后取脑进行蛋白质(TPRO)、乙酰胆碱脂酶(AChE)及一氧化氮(NO)指标测定。结果①子鼠体重随剂量的增加而下降,C组下降明显,与对照组比较差异有统计学意义(P<0.05),脑体比各组之间差异无统计学意义(P>0.05)。②水迷宫实验结果显示:B组、D组与对照组比较游泳时间缩短,错误次数减少(P<0.05);小鼠避暗实验结果显示各剂量组的潜伏期较对照组延长,而错误次数减少,但差异无统计学意义。③TPRO随剂量的增加而增高,D组较0组和A组明显增高,差异有统计学意义(P<0.05);AchE各组之间差异无统计学意义;NO含量A组较C组有明显增高(P<0.05),较B、D组差异有统计学意义(P<0.01)。结论在小鼠胚胎期及婴幼期补充胡麻籽油可提高脑内蛋白质含量,促进小鼠学习记忆能力,可引起脑内NO含量的变化。
Objective To explore the effects of α-linolenic-acid （α-LNA）-rich linseed oil on brain development and function of filial generation mice. Methods Forty-five sexually mature mice （female/male=2：l） were reared together. The pregnant mice were randomly divided into the negative control group and three experimental groups based on their bodyweight. The pregnant mice received 2g （group A）,5g （group B）, 10g （group C） /kg.bw or none （control group） of linseed oil gavage until execution at the weaning of their whelps, which were caged separately by sex. All the male filial mice were sacrificed immediately for brain and body weight measurements. The female filial mice in group C was further divided into a new group C and group D. Groups A,B and C were given once-daily linseed oil in doses described above,while group D received no linseed oil. At the end of fifth week,the mice underwent dark-escaping test,water maze test;removal of brain tissues for determination of biochemical indices such as TPRO, AChE and NO. Results （1）The body weights of filial generation decreased with higher doses of linseed oil, particularly those of group C,in remarkable contrast to the control group （P〈0.05）. No difference in brain/body weight ratio was observed across the groups （/）〉0.05）. （2）Water maze test indicated shortened time of swimming and less number of errors in groups B and C as compared with the control group （P〈0.05）; Phototaxis test showed prolonged dark-escaping latency and less number of errors in experiment groups compared with control group,but with no significant difference. （3） TPRO increased with the doses,appearing statistically higher in group D as compared with group A and controls （P〈0.05）. Difference in AChE was unremarkable among all groups. The level of NO in group A was higher when compared with group C （P〈0.05）,and significantly higher when compared with groups B and D （P〈 0.01 ）. Conclusion Supplementary linseed oil given to fetal and i
出处
《中国药物与临床》
CAS
2006年第10期733-735,共3页
Chinese Remedies & Clinics
基金
山西省卫生厅科技攻关基金资助项目(200125)
关键词
Α-亚麻酸
记忆
神经递质
亚麻籽油
Alpha-linolenic acid
Memory
Neurotransmitters
Linseed oil
