摘要
目的:探讨实时荧光定量聚合酶链反应检测非淋病性尿道炎、宫颈炎等疾病中解脲支原体(Uu)感染的准确性及其在实验室诊断中的应用价值。方法:将临床116例标本分别用实时FQ-PCR检测及常规培养法同时检测,并对两种方法的检测结果进行分析比较。结果:116例临床标本,Uu培养阳性49例,阳性率为42.2%,实时FQ-PCR阳性47例,阳性率为40.5%。Uu培养阳性的49例标本中,实时FQ-PCR法阳性46例,二者诊断符合率为96.6%,经卡方检验两种方法无显著差异(χ2=0.25,P>0.05)。实时FQ-PCR相对于培养法的敏感性为93.9%,特异性为98.5%,两法检测Uu的效率基本相当。结论:实时FQ-PCR法不仅能对标本中DNA模板拷贝数准确定量,而且比常规培养法更简便、快捷,但不能用于判定治愈。
Objective： To evaluate the accuracy and clinical application of real - time fluorescence quantitative PCR （real - time FQ- PCR） in detection of ureaplasma urealyticum （Uu） DNA in non - gonococeal urethritis and cervicitis. Methods： DNA of Uu in 116 samples was detected by the real - time FQ - PCR and the results were compared with routine culture.. Results： Forty - seven cases were. positive by real - time FQ - PCR with a positivity rate of 40.5 %. Forty- nine cases were positive by routine culture with a positivity rate of 42.2%. No significant difference between the two methods was found. Compared to the culture, the sensitivity and specificity of real - time FQ- PCR were 93.9% and 98.5%, respectively. The agreement between the two methods was 96.6%. Conclusion： The real - time FQ - PCR is a simpler, more rapid and more accurate method for the detection of Uu than culture method, but it cannot be used in determination of clinical cure of the infections.
出处
《中国麻风皮肤病杂志》
2006年第10期825-827,共3页
China Journal of Leprosy and Skin Diseases
关键词
解脲脲原体
实时荧光定量聚合酶链反应
培养
ureaplasma urealyticum
real- time fluorescence quantitative polymerase chain reaction
culture
