摘要
目的在大鼠肾系膜细胞上观察黄芪甲苷与苄达赖氨酸合用对细胞外基质(ECM)的影响并探讨其机制。方法大鼠系膜细胞在含不同药物的培养液中孵育后,放免法测定Ⅳ型胶原和层粘蛋白含量;以流式细胞仪测定细胞周期;RT-PCR法测定肾皮质TGFβ-1 mRNA的相对含量;比色法测定各抗氧化指标。结果高剂量ASI与高剂量BDL合用组(AH+BH组)和低剂量ASI与低剂量BDL合用组(AL+BL组)的Ⅳ型胶原和转化生长因子(TGFβ-1)mRNA相对含量较高糖组降低,高剂量ASI组(AH组)和高剂量BDL组(BH组)分别与AH+BH组相比差异都有显著性。AH+BH组和AL+BL组的S期细胞比例分别有不同程度的升高,与单用ASI组和单用BDL组相当。AH组和BH组的总抗氧化能力(T-AOC)水平和谷胱甘肽过氧化物酶(GSH-PX)活性分别与AH+BH组相比差异有显著性。结论合用ASI和BDL可增强它们的降低TGFβ-1水平、抑制Ⅳ型胶原生长和抗氧化的作用;从而增强了对高糖引起的ECM增生的抑制作用。
Aim To investigate the effects of co-administration of Astragalus Saponin Ⅰ （ ASI ） and bendazac lysine（BDL） on hypertrophy of cultured rat mesangial cells and its mechanism. Methods The levels of collagen IV and laminin, the percentages of cells in S phase, the relative quantity of transforming growth factor β1 （TGF-β1） mRNA and indexes of oxidative status were assayed after the cells were incubated in different agents for 36 h. Results The percentage of S phase cells in high glucose group（HG） was greatly decreased while those of vitamin E group （ VE ） and coadministration groups were increased. The relative quantity of TGF-β1 mRNA and the collagen IV level in co-administration groups were significantly decreased, and the levels of total anti-oxidative capability （T-AOC）, activity of catalase （CAT）, GSH-PX, and SOD were greatly increased. Furthermore, the significant differences were found between low ASI （AL） group, low BDL （BL） group and co-administration of low ASI and low BDL （ AL ＋ BL） group for TGF-β1 mRNA, T-AOC and GSH-PX; the high ASI group （AH）, high BDL group （BH） and co-administration of high ASI and high BDL group （ AH ＋ BH） for TGF- β1 mRNA and collagen IV, respectively. Conclusion Co-administration of ASI and BDL has synergetic effects on regulating TGF-β1, collagen Ⅳ, and radical oxidative stress, therefore, is beneficial to protecting rat mesangial cells against hypertrophy.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2006年第10期1237-1241,共5页
Chinese Pharmacological Bulletin
关键词
黄芪甲苷
苄达赖氨酸
大鼠肾系膜细胞
细胞外基质
astragalus saponin Ⅰ
bendazac lysine
rat mesangial cell
extracellular matrix
