摘要
目的建立非水溶性抗生素原料的微生物限度检查方法并对其进行验证。方法抗生素原料,用经干热灭菌的5%吐温-80作湿润剂,pH7.0氯化钠蛋白胨缓冲液稀释,上清液500 r.m in-1离心,离心后的上清液用薄膜过滤器进行薄膜过滤并用0.1%蛋白胨冲洗液冲洗,薄膜置营养琼脂培养平板上培养。结果此方法有效地解决了这类非水溶性原料在水中漂浮以致前处理困难的问题,并较为完全地消除了具有这类强抗菌活性原料对微生物限度检查的内在干扰。结论该方法适合于非水溶性抗生素原料的微生物限度检查。
OBJECTIVE To provide a method of microbial limit test on insoluble antibiotic material. METHODS 5% Twain 80 sterilized by dry heat air and pH 7.0 sodium chloride peptone solution were added to antibiotic material. The upper solution was trans-feted into centrifugal tube and centrifugated at 500 r·min^-1, and then the supernatant was filtered with the membrance, and wash the filter with 0.1% peptone washing liquid, cultivate the thin-layer on nutrition agar slab. RESULTS This method effectively solved the problem of floating and completely eliminated the disturbance to microbial limit test of it. CONCLUSION The method is suitable to the microbial limit test for the insoluble antibiotic material.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2006年第5期394-396,共3页
Chinese Journal of Modern Applied Pharmacy
