摘要
目的研究脑内局部给药建立颞叶癫疒间(EP)模型的方法。方法通过立体定向技术向大鼠海马局部注射海人酸(KA)4μg/kg,观察注射后大鼠的行为学、脑电图和病理学改变。结果KA注入海马后大鼠表现为凝视、湿狗样抖动、咀嚼运动、点头、肢体阵挛等;随后出现阵发性旋转、向上窜跳、四肢抽搐,反复间歇性发作,约10h后发作停止。以后每周约自发性发作1～3次,主要为Ⅱ～Ⅳ级发作。EEG记录到大脑皮质丛集放电,棘波或尖波持续发放。光镜下KA致疒间鼠海马结构的CA1区和CA3区锥体细胞变性及坏死,实验侧CA3区神经元丢失尤为明显。结论大鼠脑内局部注射KA可成功建立颞叶EP模型,其行为症状、电生理及海马病理改变类似人颞叶EP,是很好的研究颞叶EP的工具。
Objective To explore a better method to establish temporal lobe epilepsy model by administrating drug in brain region. Methods Kainie acid （KA） 4μg/kg was injected into rat＇s hippoeampus by stereotaetie operation. The rat＇s behavior, EEG and pathological changes were observed. Results After the rat＇s hippoeampus injected with KA, staring, wet-dog shakes, mastieatory movement and elonus of limbs occurred successively. The seizures were paroxysmal with rotation, unsettled state of jump and tie of limbs. The rats＇ behavior gradually recovered to normal after 10 hours. Then the spontaneous seizure （ mostly rating 2 - 4） oeeurred 1 - 3 times every week. Cluster electric discharge, spike waves and sharp waves were recorded in cerebral cortex. KA-treated rats could result in hippoeampal CAl and CA3 fields neuronal degeneration and necrosis, especially significant neuron loss was observed in the CA3 field of KA injected ipsilateral side. Conclusions Injection KA in brain region of rat can establish temporal epilepsy model. The symptom, eleetrophysiology and pathological changes of temporal lobe epilepsy in the rat model are almost the same as those in human being. The KA induced rat model is an ideal tool to research human temporal lobe epilepsy.
出处
《临床神经病学杂志》
CAS
北大核心
2006年第5期362-364,共3页
Journal of Clinical Neurology
关键词
颞叶癫痫
模型
大鼠
海人酸
temporal lobe epilepsy
model
rat
kainie aeid
