摘要
目的观察缺氧复氧对大鼠小肠上皮细胞(intestinal epithelial cell,IEC)-6培养液中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量及细胞内Ca^(2+)浓度和线粒体膜电位的影响,探讨复方中药-紫芪方对IEC-6肠上皮细胞缺氧复氧损伤的保护作用及机制。方法复制IEC-6细胞缺氧复氧损伤模型,采用紫外分光法测定缺氧复氧后及紫芪方血清治疗后细胞培养液中SOD活性及MDA含量；采用激光共聚焦显微镜测定细胞Ca^(2+)浓度和线粒体膜电位变化。结果细胞缺氧复氧损伤后,可导致细胞培养液中SOD活性显著降低、MDA含量显著升高、线粒体膜电位明显降低及细胞内Ca^(2+)浓度明显升高(P＜0．01)。紫芪方药物血清治疗后,可提高细胞培养液中SOD活性并降低其MDA含量,同时稳定了线粒体膜电位并降低细胞内Ca^(2+)浓度(P＜0．05)。结论缺氧复氧可导致IEC-6肠上皮细胞损伤；紫芪方血清对IEC-6小肠上皮细胞缺氧复氧损伤具有显著的保护作用。
Objective To observe the effect of hypoxia reoxygenation on activity of superoxide dismutase （SOD） and MDA content as well as [ Ca^2＋ ] concentration and mitochondria membrane potential of intestinal epithelial cell-6 （IEC-6） in IEC culture medium and explore the protective effect and mechanism of serum containing Ziqi-liquid （ a preparation of Chinese herbal medicine） on hypoxia reoxygenation damaged IEC-6. Methods Hypoxia reoxygenation damage model of IEC-6 was made. SOD activity and MPA content in IEC-6 culture medium were determined by ultraviolet spectrometry after hypoxia reoxygenation and treatment with Ziqi-liquid. Meanwhile, MMP changes and [ Ca^2＋ ], concentration were detected by laser scanning confocal microscopy. Results After hypoxia reoxygenation, SOD and MMP were significantly decreased, but MDA content and [ Ca^2＋],concentration significantly increased （ P 〈 0.01 ）, and significantly facilitated by serum eontaining Ziqi-liquid. Conclusion Hypoxia reoxygenation can damage IEC-6, but the serum containing Ziqi-liquid has significant protective effect on it.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2006年第10期763-766,共4页
Chinese Journal of Trauma
基金
国家重点基础研究发展规划资助项目(G1999054203)
关键词
休克
上皮细胞
小肠
细胞低氧
细胞保护
紫芪方
Shock
Epithelial cells
Intestines
Cell hypoxia
Cytoprotection
Ziqiliquid
