摘要
目的建立肿瘤相关性抑郁动物模型,并观察其生物学行为及生化指标。方法SD大鼠随机分为四组,分别于接种肿瘤细胞前后采用长期不可预见性中等强度应激造成分养大鼠抑郁症模型,制备大鼠肿瘤相关性抑郁模型。采用开场试验检测大鼠的行为性抑郁,利用荧光分光光度法测定海马5-羟色胺(5-HT)含量,同时监测体质量、皮下肿瘤大小,糖水消耗试验。结果大鼠接受慢性应激后出现显著的抑郁,表现为在中央区域活动显著减少,总的活动路程减少,休息时间延长和活动时间缩短,在停止应激后的第12d抑郁仍维持较高程度。抑郁大鼠糖水消耗量减少。抑郁模型组海马5-HT分别为(239.6±11.4)、(237.9±14.4)、(196.7±17.2)ng/g均低于肿瘤对照组(292.9±20.2)ng/g;4组大鼠均生长肿瘤。结论本实验成功地构建了肿瘤相关性抑郁的动物模型,荷瘤鼠存在明显的行为性抑郁及脑内神经递质的改变,是研究抑郁对肿瘤发生、发展、预后影响的理想平台。
Objective To establish an animal model of tumor-associated depression and observe their biological behaviors and biochemical indices. Methods Four groups of SD rats kept in separate cages were subjected to tumor cell inoculation with or without chronic unpredictable moderate stress administered before or after the inoculation. The depressive behaviors of the rats were examined by open-field test, and the concentration of 5-HT in the hippocampus was measured by spectrophotofluorometry. The body waght of the rats and volume of the implanted tumor were monitored and sugar water test was performed. Results The rats subjected to chronic stress displayed significant depression, manifested by reduction in movement in the central area and total movement distance with prolonged resting time and shortened time of activity, These rats maintained high levels of depression even 12 days after withdrawal of chronic stress. Compared with the control group, the depressive rats showed obviously reduced sugar water consumption and hippocampal 5-HT level. Tumors of different sizes were observed in all rats in the 4 groups. Conclusion A rat model of tumor-associated depression is established, and the tumor-bearing rats exhibit obvious depressive behaviors and reduced level of neural substance （5-HT）, which provides a good basis for studying the association of depression with tumorigenesis, ,progression and prognosis of tumor.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2006年第10期1513-1514,1517,共3页
Journal of Southern Medical University
关键词
肿瘤
抑郁
动物模型
tumor
depression
animal model
