摘要
在PEG-6000胁迫条件下,以微管蛋白基因为内参基因、水通道蛋白基因ZmPIP1-1和ZmPIP1-2为检测基因,采用半定量逆转录聚合酶链式反应(RT-PCR)体系检测它们在玉米根系中的表达情况。实验结果是:胁迫条件下,ZmPIP1-1的表达量在杂交F1代‘户单4号’(抗旱)和母本‘天四’(抗旱)根系中增多,它的表达量与品种的抗旱性呈正相关,并且胁迫不同时间段它的表达量有差异;而ZmPIP1-2在3个玉米品种的不同水分处理条件下,表达量均没有明显变化。这提示,水分胁迫条件下根系中某些种类的水通道蛋白基因的表达量增多,并且与品种的抗旱性有关;而另一些水通道蛋白基因的表达不受水分亏缺的影响。
To investigate the role of ZmPIP1-1 and ZmPIP1-2 in water uptake of roots and drought resistance of crops, semi-quantitative PCR was used to examine the expression of ZmPIP1-1 and ZmPIP1-2 in root systems of different maize genotypes under water deficit. These genotypes showed different resistance to water shortage under field conditions. The reference gene to target genes was tubulin. Maize seedlings were grown by hydroponics in a growth chamber. Water deficit was imposed on the seedlings with PEG-6000. The result showed that ZmPIP1-1 was up-regulated under water deficit in root systems of plants of the filial generation ‘Hudan 4＇ and the mother line ‘Tiansi＇, which were resistant to water shortage, but there was no noticeable up-regulation of ZmPIP1-1 in the root systems of the father line ‘803＇, which was sensitive to water deprivation. The result also showed that the extent of up-regulation was positively correlated with drought resistance of maize （Fig.3）. On the other hand, the expression of ZmPIP1-1 showed different degrees of tendency after different duration of water stress in the root systems of the maize seedlings of different genotypes. The result showed that ZmPIP1-2 was identically expressed in three different species of maize and under different water conditions. The results support the theory that the intercellular water transport contributes to increased water uptake in root systems under water deficit by up-regulating the number of some kinds of aquaporins. The increases amount of transcripts of aquaporins is positively correlated to drought resistance of plant varieties. But not all kinds of number of aquaporins is up-regulated during water shortage, some kinds of aquaporins are identically expressed under water deficit conditions and well watered conditions.
出处
《植物生理与分子生物学学报》
CAS
CSCD
北大核心
2006年第5期557-562,共6页
Journal Of Plant Physiology and Molecular Biology
基金
国家自然科学基金(No.30571127)
中国科学院知识创新工程重要方向项目(No.KZCX3-SW-444)
中国科学院水土保持研究所领域前沿科研专项经费(No.SW05101)资助~~
