摘要
荧光法是利用大肠菌群能分解4-甲基伞型酮-β-D半乳糖苷,使4-甲基伞形酮游离,在波长366nm的紫外灯照射下可呈现蓝色荧光。本实验将大肠菌群所需的营养成分经过浓缩、真空干燥制成一次性检测试管,并针对制备的检测试管进行了特异性、灵敏度、稳定性实验和蔬菜中大肠菌群的检测方法比较研究。结果表明该方法快速、简便、准确、稳定,可应用于蔬菜中大肠菌群的快速检测。
The fluorescence method utilizes the principle that coliform decomposes 4-mehtyumbelliferyl- β -D-galactoside,causing 4-mehtyumbellifery dissociated, which will emit blue fluorescence when being illuminated with 336nm UV light. In the experiment, the nutriments needed by coliform are concentrated and vacuum-dried to produce disposable testing cuvettes and using these testing cuvettes, specificity, sensitivity and stability tests as well as a comparative study against other inspecting methods of coliform in vegetables are performed. The results show that this method is fast, simple, accurate and stable and is applicable for fast detection of coliform in vegetables.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第10期480-484,共5页
Food Science
基金
福建省"十五"重大科技攻关项目(2003Y005-06)
关键词
荧光法
大肠菌群
蔬菜
fluorescence method
coliform
vegetable
