摘要
目的:观察腺病毒介导的人心肌营养素(humancardiotrophin-1,hCT-1)对大鼠不完全性颈脊髓损伤(spinalcordinjury,SCI)后可塑性变化、功能恢复和运动诱发电位的作用。方法:在立体定位仪的引导下,致伤大鼠双侧红核和颈3节段皮质脊髓背侧束SCI局部注射hCT-1重组腺病毒载体,并采用荧光免疫组织化学检测其表达。采用改进后的大鼠前肢食物小球抓取动作评分标准和运动诱发电位评定大鼠前肢功能恢复情况。生物素化葡聚糖胺示踪未受损皮质脊髓腹侧束(ventralcorticospinaltract,vCST)的出芽情况。结果:SCI局部高效表达的hCT-1诱导vCST出芽增加,同时伴有大鼠前肢运动功能和运动诱发电位P1、N1波波幅的恢复。结论:腺病毒介导的hCT-1在SCI局部的高效表达可增加vCST的出芽数量,并促进运动功能的恢复。
Objective To observe the effects of adenovirus-mediated human cardiotrophin-1 （hCT-1） gene transfer on functional recovery, neural plasticity and motor-evoked potential （MEP） after incomplete cervical spinal cord injury （SCI） in rats. Methods Stereotaxically, bilateral red nuclei and cervical dorsal corticospinal tract （dCST） were destroyed and hCT-1 recombinant adenovirus vector was applied to the SCI site. The expression of hCT-1 was detected by fluorescent immunoehemistry, and the rats＇ forelimb performance was evaluated by forelimb food pellet retrieval task and MEP. Biotin dextran amine was used to trace the Sprouting of intact ventral corticospinal tract （vCST）. Results hCT-1 was efficiently expressed at the SCI site, promoting the recovery of rats＇ forelimb function and normalizing the amplitude of N1 and P1 in MEP. Also, the number of vCST sprouting increased. Conclusions After incomplete cervical SCI in rats, adenovirus-mediated hCT-1 gene transfer can enhance the sprouting of intact vCST, which results in the recovery of forelimb function.
出处
《实用医学杂志》
CAS
2006年第20期2338-2341,共4页
The Journal of Practical Medicine
基金
国家自然基金面上项目资助项目(编号:30300075)
关键词
脊髓损伤
心肌营养素
出芽
Spinal cord injuries Cardiotrophin-1 Sprouting
