摘要
目的研究心力衰竭(heartfailure,HF;简称心衰)幼鼠心肌结缔组织生长因子(connectivetissuegrowthfactor,CTGF)表达及苯那普利的调节作用。方法采用腹主动脉缩窄术建立幼鼠HF模型,术后6周经高频超声筛查将符合心衰标准的幼鼠随机分为HF组和苯那普利治疗组,另设假手术组。苯那普利治疗组灌胃给药4周,术后10周时行高频超声、心肌重量分析、免疫组化和逆转录-聚合酶链反应分别检测心肌功能、心室重构程度、心肌CTGF表达。结果10周时于3组中各随机抓取15只幼鼠进行检测。与假手术组比较,HF组左室舒张末期内径(LYEDD)、左室收缩末期内径(LVESD)、室间隔舒张末期内径(IVSTd)、室间隔收缩末期内径(IVSTs)、左室后壁舒张末期厚度(LVPWTd)、左室后壁收缩末期厚度(LVPWTs)、左心室相对质量(LVRW)、右心室相对质量(RVRW)均升高(P<0.01),而短轴缩短率(FS)、射血分数(EF)降低(P<0.01).CTGF免疫阳性细胞增多,CTGFmRNA表达亦高[(0.609±0.065)vs(0.117±0.011),P<0.01]。苯那普利组与HF组相比LVESD,IVSTd,IVSTs,LVPWTd,LVPWTs,LVRW,RVRW降低(P<0.01),FS、EF升高(P<0.01),CTGF免疫阳性细胞减少,CTGFmRNA表达下降[(0.441±0.053)vs(0.609±0.065),P<0.01]。结论HF幼鼠心肌CTGF表达明显上调,苯那普利可部分抑制HF心肌CTGF的表达,从而抑制HF时心室重构的发生,改善心脏功能。
Objectives Ventricular remodeling is an important pathologic progress in almost all end stage heart failure （ HF）, and it is characterized by ventricular thickening and cardiac fibrosis with poor prognosis. The connective tissue growth factor （ CTGF）, a new growth factor with multi-function, has an important role in fibrosis of tissue and organs. It has been demonstrated that angiotensin-converting enzyme inhibitor（ACEI） can prevent the development of cardiomyocyte from remodeling and improve cardiac function. Researchers try to test the hypothesis that cardiac function improvement attributable to ACEI is associated with inhibiting expression of CTGF in patients with HF. The aim of this study was to observe changes in CTGF expression in cardiomyocyte of young rats with HF and effect of benazepril on CTGF. Methods The animal model of HF was established by constriction of abdominal aorta. Five weeks old rats were randomly divided into 3 groups after 6 weeks of operation ： （ 1 ） HF group without treatment （ n = 15） ; （2） HF group where rats were treated with benazepril （n = 15） ; （3） sham-operated group （n = 15） where rats were administered benazepril through direct gastric gavage. After 4 weeks of treatment, the high freguency ultrasound was performed. The expression of CTGF was detected by immunohischemistry and semiquantative reverse transcription-polymerase chain reaction. Results Compared with the sham-operated group, left ventricular diastolic dimension （LVEDD）, left ventricular systolic dimension （LVESD）, interventricular septal thichness at end-diastole （IVSTd）, interventricular septal thickness at end-systole （IVSTs）, left ventricular posterior wall thickness at end-diastole （LVPWTd） , left ventricular posterior wall thickness at end-systole （ LVPWTs）, left ventricular relative weight （ LVRW）, and right ventricular relative weight （RVRW） were all increased （P 〈0. 01 ） , but ejection fraction （EF） and fractional shortening （FS�
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2006年第10期733-737,共5页
Chinese Journal of Pediatrics
