摘要
目的:建立广金钱草黄酮对照品6-C-葡萄糖-8-C木糖洋芹素(vicen in-2)的反相高效液相色谱制备方法。方法:结合萃取、大孔树脂柱层析、重结晶等方法,用反相高效液相色谱法对广金钱草的乙醇提取物进行分离纯化,以HPLC对分离产物进行纯度和含量测定。结果:从广金钱草中分离纯化出黄酮对照品vicen in-2纯度可以达到98.5%以上。结论:该方法简单、准确、重现性好,产品纯度高,可作为中药药效物质基础研究中的对照品。
AIM ： To develop an RP-HPLC method for preparing the reference substanc of vicenin-2 in Desmodium styracifolium. METHODS： Ethanol-extract of desmodium styracifolium was isolated and purified by RPHPLC combining solvent extraction with column chromatography and recrystalliztion. The purity and content of vicenin-2 were identified by HPLC. RESULTS： The flvonoids were completely separated under this chromatographic condition. The purity of the reference substance was 99.0% or above. CONCLUSION ： The method is simple, accurate, better on repeatability, and effective to yield high-purity product. It can be used as reference substance for the research of herbal medicine.
出处
《中成药》
CAS
CSCD
北大核心
2006年第10期1491-1493,共3页
Chinese Traditional Patent Medicine
