摘要
从食物垃圾中分离到1株乳酸高产菌株TD175,该菌株在含100 g/L葡萄糖的发酵培养基中,经72 h发酵,可产生78.56 g/L的乳酸.根据形态和生理生化特征,将菌株TD175初步鉴定为乳杆菌属(Lactobacillussp.)的细菌,其16S rDNA序列与乳杆菌MR2菌株、植物乳杆菌(L.plan tarum)和戊糖乳杆菌(L.pen tosus)的相似性最高,均达到99%.菌株TD175经过耐酸选育得到的新菌株TD175-1的乳酸产量提高了10.7%.菌株TD175-1能促进食物垃圾的乳酸发酵,厌氧发酵48 h,产生29.65 g/L的乳酸,比不接种的自然发酵高34.3%.
A strain of high -yield lactic acid bacterium, designated as TD175, was isolated from food waste. The strain TD175 produced 78. 56 g/L of lactic acid from 100 g/L of glucose after 72 h fermentation. Based on its morphology and physio-biochemical properties, the strain TD175 was tentatively identified as LactobaciUus sp.. Analysis of 16S rDNA sequence showed that TD175 had high homology （99%） with Lactobacillus sp. MR2, L. plantarum and L. pentosus. An acid-tolerance strain TD175 - 1 was obtained by breeding of the strain TD175. Lactic acid output for the strain TD175 - 1 was 10. 7% higher than the strain TD175. The strain TD175 - 1 was capable of enhancing lactic acid fermentation of food waste, with 29. 65 g/ L of lactic acid produced after 48 h of anaerobic fermentation, which was 34. 3% higher than the spontaneous fermentation without inoculum.
出处
《哈尔滨工业大学学报》
EI
CAS
CSCD
北大核心
2006年第9期1483-1486,1495,共5页
Journal of Harbin Institute of Technology
基金
国家自然科学基金资助项目(50278024)
黑龙江省自然科学基金资助项目(E0316)
关键词
乳酸
乳酸细菌
发酵
鉴定
lactic acid
lactic acid bacterium
fermentation
identification
