摘要
目的了解大肠埃希菌产质粒介导AmpC酶的现状及其基因型。方法收集任一第三代头孢菌素和头孢西丁耐药的大肠埃希菌临床分离株42株,以改良三维试验检测超广谱β-内酰胺酶(ES-BLs)和AmpC酶,用碱裂解法提取质粒,应用多重聚合酶链反应(multiplexpolymerasechainreaction,MPCR)对AmpC酶的基因进行检测,对PCR扩增阳性的样本进行基因测序以确定基因型。结果42株大肠埃希菌中,经三维试验检测,单纯产ESBLs者18株(42.8%),单纯产质粒AmpC酶者4株(9.5%),同时产质粒AmpC酶与ESBLs者1株(2.3%),5株AmpC酶三维试验阳性菌株,经PCR扩增其中4株扩增到了清晰的条带,1株扩增为阴性,PCR产物经测序分析其基因型均为DHA-1型。结论大肠埃希菌临床分离株产ESBLs率较高,并己经出现了质粒介导的AmpC酶,其基因型为DHA-1型。
Objective To investigate the genotypos coding plasmid-mediated AmpC in the isolates of Escherichia coll. Methods 42 strains of Cefoxitin and third-generation cephalosporin -resistant Escherichia coli were isolated from clinical specimens in our hosptial. AmpC and extened spectrum β-lactamases （ESBLs） were determined by modified three-dimensional test. Multiplex polymerase chain reaction was used to detect the genotypos of AmpC. The products of PCR were sequenced. Results Of 42 isolates,18 isolates only produced ESBLs and 4 isolates only produced AmpC. 1 isolates produced either ESBLs or AmpC. Among 5 isolates producing AmpC, The products of mPcR of 4 isolates were positive. The genotypes coding plasmid-mediated AmpC in 4 isolates all were DHA-1. Conclusion The rate of ESBLs in the isolates of Escherichia coli is very high. The genetype coding plasmid-mediated AmpC in the isolates of Escherichia coli is DHA- 1.
出处
《江西医学检验》
CAS
2006年第5期393-396,共4页
Jiangxi Journal of Medical Laboratory Sciences
基金
江西省卫生厅重大技术招标基金资助项目200211
关键词
大肠埃希菌
质粒
AMPC酶
基因型
多重PCR
Escherichia coli
Plasmid
AmpC
Genetype
Multiplex polymerase chain reaction
