摘要
目的观察脓毒症大鼠心肌组织的病理改变以及氧化应激相关指标的变化。方法38只大鼠随机分为两组。以盲肠结扎穿孔术(CLP)建立脓毒症模型,同时设立假手术对照组,24小时后观察两组大鼠心肌病理变化并分别测定其血清心肌酶浓度及心肌组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧物酶(GSH-PX)活性、丙二醛(MDA)浓度及其抑制超氧阴离子(O2.-)、羟自由基(OH.-)的能力。结果脓毒血症组大鼠心肌病理学改变包括间质充血水肿、炎性细胞浸润,与对照组相比,脓毒血症组大鼠血清心肌酶水平,心肌组织中SOD、GSH-PX、MDA浓度及其抑制O2.-的能力在24小时后均有明显升高。结论氧化应激参与脓毒症大鼠心肌损伤的发生。
Objective To investigate the pathological changes and alterations of parameters associated with oxidative stress in myocardium of rats with sepsis. Methods Thirty-eight Wistar rats were randomly divided into two groups： sepsis model group （CLP group： cecal ligation and puncture to establish sepsis model, n= 25） and control group （sham procedure, n= 13）. 24 hours later after procedure, pathologic changes in heart tissue of all animals were observed. Meanwhile serum concentrations of myocardial enzyme and cardiac superoxide dismutase （SOD）, glutathione peroxidase（GSH- PX） and malondialdehyde （MDA） were detected in both groups, the capability of inhibition of superoxide anion, hydroxyl radicals in the heart tissue were also measured. Results The pathologic changes of heart tissue in septic rots included edema, congestion and inflammatory cells infiltration in mesenchyma, no pathological damage was seen in control group rats. Compared with controls, serum concentration of myocardial enzyme： cardiac SOD, GSH-PX, MDA and the capability of inhibition of superoxide anion were significantly increased in septic rats 24 hours post-operation （ P 〈 0.01 ）. Conclusions Oxidative stress involves in the pathogenesis of the myocardial injury in sepsis.
出处
《心脑血管病防治》
2006年第5期294-296,共3页
CARDIO-CEREBROVASCULAR DISEASE PREVENTION AND TREATMENT
基金
卫生部科研基金-浙江省医药卫生重大科研项目(No.WKJ2005-2-035)
关键词
氧化应激
脓毒症
心肌损伤
大鼠
Oxidative stress
Sepsis
Myocardial injury
Rats
