摘要
目的探讨靶向surv iv in基因阻断后mRNA表达量的变化,以及在体外对肿瘤细胞的抑制作用。方法应用分子克隆技术构建siRNA真核表达载体Pgenesil/sur;通过脂质体转染腺样囊性癌(salivary adeno id cystic carcinom a,SACC)细胞。采用RT-PCR技术检测转染前后surv iv in mRNA表达量以及表达抑制率。结果转染后surv iv in mRNA表达水平x-±s(0.42±0.10)较转染前surv iv in mRNA表达水平(1.56±0.12)明显减低,P<0.05;在mRNA水平其表达抑制率为73.1%。转染空质粒载体Pgenesil-1 surv iv in mRNA表达水平(1.35±0.13)与空白对照组比较无统计学差异(P>0.05)。结论靶向surv iv in siRNA能阻断肿瘤细胞surv iv in基因mRNA表达,并有效抑制体外培养的肿瘤细胞SACC-83的增殖作用。
Objective To explore the expression of mRNA and the inhibition to the salivary adenoid cystic carcinoma cell in vitro after the survivin gene has been repressed. Methods：To construct siRNA by molecular cloning technique. Eukaryon express the vector Pgenesil. Then transfected into salivary adenoid cystic carcinoma cells by using liposome. The expression of survivin mRNA and the inhibition to the turnout cell in vitro were detected by RT-PCR respectively. Result ：The expression of survivin mRNA was 0.42±0. 10,which was significantly lower than that of the control group（1.56±0. 12,P〈 0. 05）. The expression inhibition rate in the mRNA level was 73.1%. Conclusion： The survivin siRNA can represss the mRNA expression of salivary adenoid cystic carcinoma and repress the generation of the salivary adenoid cystic carcinoma cells in vitro utility.
出处
《现代检验医学杂志》
CAS
2006年第5期29-31,共3页
Journal of Modern Laboratory Medicine
