摘要
目的:同步联检慢性心力衰竭(CHF)患者血中9种细胞因子浓度并探讨其临床意义。方法:运用蛋白芯片技术联检64例慢性心力衰竭患者及40例健康对照组血清或血浆中的9种细胞因子含量,选择其中3种细胞因子与经典的酶联免疫双抗体夹心吸附实验(ELISA法)对照。结果:ELISA法和蛋白芯片法联检细胞因子相关性较好,2种方法同时检测血清中基质金属蛋白酶-9(MMP-9)及可溶性CD40L(sCD40L)水平,差异无统计学意义(P>0·05);CHF组与对照组比较:血清中的MMP-9、sCD40L、C-反应蛋白、心脏型脂肪酸结合蛋白及白细胞介素-6、血浆中的BNP前体N-终端、可溶性血管细胞黏附分子-1(sVCAM-1)、内皮素-1和白细胞介素-8浓度升高,差异有统计学意义(P<0·01,P<0·05);随着心功能级别的升高,患者血清中的MMP-9、sCD40L水平明显升高,心功能Ⅳ级>Ⅲ级>Ⅱ级(P<0·01),心功能Ⅳ级与心功能Ⅱ+Ⅲ级患者的sVCAM-1比较,差异有统计学意义(P<0·05);血中MMP-9、sCD40L及sVCAM-1浓度与心功能分级显著正相关(r=0·640,r=0·818,r=0·845,P<0·001),随着心功能下降程度的增加,血中MMP-9、sCD40L及sVCAM-1水平明显升高(P<0·01,P<0·05)。结论:CHF患者血中9种细胞因子水平异常,其中MMP-9、sCD40L、sVCAM-1浓度与心功能的严重程度密切相关,提示上述细胞因子参与和促进不同病因CHF的发生、发展。
Objective： To explore the significance of united detection of nine-cytokines in patients of chronic heart failure（CHF） with protein chips. Method：In view of united detection of serum or plasma levels of 9 cytokines in 64 CHF cases and 40 health cases as control. Result：Levels of serum MMP-9 and sCD40L by protein chip were well correlated to that of by ELISA（ P 〉0.05）. The levels of serum MMP-9, sCD40L, H-FABP, CRP, IL-6, and plasma NT-proBNP,sVCAM-1 ,ET-1 , IL-8 in the CHF cases were much higher than those of the control cases （ P 〈0.05, P 〈0.01）. The levels of serum MMP-9, sCD40L, sVCAM-1 increased significantly in CHF cases with the increased grades of cardiac function, and the levels of serum MMP-9, sCD40L were much higher in NYHA Ⅳ than those of NYHA Ⅲ（ P 〈20.05）. The levels of serum MMP-9,sCD40L were much higher in NYHA Ⅲ than those of NYHA Ⅱ （ P 〈20.05）. The level of sVCAM-1 was much higher in NYHA Ⅳ than that of in NYHA Ⅱ ＋ Ⅲ Levels of serum MMP-9,sCD40L and plasma sVCAM-1 had positive relationship with grades of cardiac function（ r =0. 640, r =0. 818, r =0. 845, P〈0.01）. The levels of serum MMP-9,sCD40L and plasma sVCAM- 1 increased significantly in CHF cases with the increased grades of cardiac function. Conclusion：The levels of circulating 9 cytokines are abnormal and they may play a pathogenic role in the development and progression of CHF. Levels of MMP-9, sCD40L, and sVCAM-1 have closely relationship with cardiac function status, they may play a pathogenic role in the development and progression of CHF.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2006年第10期603-606,共4页
Journal of Clinical Cardiology
