摘要
目的:对8种肿瘤相关抗原(tumor-assoc iated antigen,TAA)用于检测肝癌的诊断价值进行评价,从而寻找一种真实可靠的肝癌早期诊断方法。方法:利用酶联免疫吸附试验(ELISA)检测原发性肝癌患者血清和正常血清中8种TAA抗体(包括C-myc,Cyc linB1,IMP1,Koc,P16,P53,P62,Survivin),利用流行病学方法对各抗原检测结果的真实性进行评价。结果:每种抗原检测结果单独进行判断时,大多数指标的灵敏度偏低;对8种TAA进行不同的组合,结果随着抗原种类的增多,诊断的灵敏度随之增加,8种抗原联合检测的灵敏度达到了81%,特异度也达到了86.7%,阳性预测值92.1%,阴性预测值70.3%,提示8种抗原并联试验检测肝癌时大大提高了诊断质量。阳性似然比6.09,说明8种抗原联合检测肝癌的临床诊断价值较高,kappa值0.78,提示该试验诊断结果与真实值之间高度一致。结论:利用8种TAA抗原联合检测肝癌,具有较高的真实性,为现场高危人群的筛检和临床中肝癌的早期诊断提供了一种新方法。
Objective：To evaluate the diagnostic value of eight tumor - associated antigens （TAAs） on hepatocellular carcinoma （HCC） and develop a valid and reliable diagnostic method on HCC. Methods：The antibodies against eight TAAs including C - myc, CyclinB1, IMP1, Koc, P16, P53, P62, Survivin in sera from patients with HCC and normal human serum were detected by enzyme - linked immunosorbent assay （ELISA）. The validity of testing result for each TAA antibody was evaluated by epidemiological method. Results：When results were judged according to any individual TAA, the majority of sensitivities rarely exceeded 20%. With the successive addition of TAAs to a final total of eight antigens, there was a stepwise increase of positive antibody reactions reaching a higher sensitivity. In parallel assay of eight TAAs, sensitivity raised at 81%, and specificity reached at 86. 7%. Positive and negative predictive values were 92. 1% and 70. 3%, respectively. It indicated that parallel assay of eight TAAs raised the diagnostic quality greatly. Positive likelihood ratio was 6. 09, which showed that the clinical diagnostic value of parallel assay of eight TAAs was high. Kappa value was 0. 78, which indicated that the observed value of this assay had high coincidence with actual value. Conclusion：This study demonstrated that a mini - array of eight TAAs might enhance antibody detection for diagnosis of HCC, the approach could be used as a screening tool for population at high risk in HCC, and also a routine test in clinical practice.
出处
《中国卫生检验杂志》
CAS
2006年第10期1160-1162,1188,共4页
Chinese Journal of Health Laboratory Technology
基金
国家自然科学基金(30471966)
河南省杰出人才创新基金(0621002500)
关键词
肝癌
肿瘤相关抗原
诊断试验
评价
Hepatocellular carcinoma（HCC）
Tumor- associated antigen（TAA）
Diagnostic test
Evaluation
