摘要
定量PCR技术以其高特异性、高灵敏性以及高自动化等特点在临床病原微生物诊断、部分遗传性疾病诊断和癌症辅助诊断等方面得以广泛应用。设计和建立符合相关标准的临床定量PCR实验室不但是相关基因扩增、检验的需要,而且是保障生物安全和保持检测结果准确性的需要。文中介绍了优化设计的单流向、一体化临床定量PCR实验室的结构布局、内部设施以及温度、噪音等人体适应性指标。
Fluorogenic quantitative polymerase chain reaction (FQ-PCR) is an emerging technique with high specificity, susceptibility, automatism and has been widely used for the detection and quantification of microorganism, some genetic diseases and cancer. The design and establishment of standard FQ-PCR laboratory are required not only by gene expansion and diagnosis but also by biosafty and veracity. The aim of the paper is to introduce the structural characteristics, instruments requirement and comfort parameters of FQ-PCR laboratory.
出处
《医疗卫生装备》
CAS
2006年第9期34-35,39,共3页
Chinese Medical Equipment Journal
基金
国家自然(30271479)
全军后勤"十一五"重点项目(06-06)资助
关键词
定量PCR
实验室:优化设计
fluorogenic quantittative polymerase chain reaction
laboratory
optimization design