摘要
目的:建立虎杖中白藜芦醇及其糖苷异构体含量的测定方法。方法:以甲醇提取目标组分,以聚酰胺薄膜为固定相,苯-甲醇-甲酸(10:5:1)为展开剂层析分离,用荧光扫描法定量,λ_(Ex)=306nm,λ_(Em)=400nm,线性参数SX=3。结果:4种组分(顺式白藜芦醇及其糖苷和反式白藜芦醇及其糖苷)的线性关系良好,相关系数为0.9942～0.9996,平均回收率为97.6%～98.6%,RSD为1.8%～3.1%。测定4个虎杖样品中反式白藜芦醇和反式白藜芦醇苷的含量分别为0.20%～0.22%和2.04%～2.14%,均未检出可定量测定的顺式异构体。结论:方法简便、快速、灵敏、测定成本低。
Objective ：To develop a method for determining resveratrol and piceid in Polygonum cuspidatum Sieb. et Zucc. by TLC fluorescence scanning, nethods：Resveratrol and piceid were extracted by methanol. The separation was accomplished on polyamide plate by development using the mobile phase benzene- methanol- formic acid （10： 5： 1）. The contents of resvertrol and piceid were determined by TLC fluorescence scanning and λEx = 306 nm, λEm = 400 nm,SX = 3. Results： The calibration curves showed good linearity and the interrelated ratio was 0. 9942 - 0. 9996. The average recoveries were 97. 6% - 98. 6% with RSD1.8% - 3. 1%. 4 samples were determined by the method. The level of trans - resveratrol and trans - piceid were 0. 20% - 0. 22% and 2. 04% - 2. 14%, respectively. Conclusion：The results show that the method is convenient ,rapid ,sensitive and low cost.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第9期1334-1336,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
薄层荧光扫描法
虎杖
白藜芦醇
白藜芦醇苷
TLC fluorescence scanning
Polygonum cuspidatum Sieb. et Zucc. , resveratrol,piceid
