摘要
目的探讨乙型肝炎患者HBV DNA含量在疾病诊治过程中的价值。方法采用荧光定量PCR技术检测568例患者血清中HBV DNA含量,比较急、慢性乙型肝炎及乙型肝炎肝硬化HBV DNA含量的差异,同时对病毒标志物进行联合检测。结果急性乙型肝炎(AH)患者血清中HBV DNA含量(2.62±1.73,拷贝/ml)显著低于慢性乙型肝炎(CH)(5.68±1.98,拷贝/ml)(P=-0.007)和乙型肝炎肝硬化(LC)患者(4.87±1.82,拷贝/ml)(P=-0.031),后二者之间无明显差别(P=0.093):HBeAg阳性者血清中HBV DNA含量(5.83±1.42,拷贝/ml)显著高于HBeAg阴性者(1.88±1.02,拷贝/ml)(P=-0.000)。结论血清HBV DNA含量与乙型肝炎的不同病程密切相关,HBeAg阳性者血清中HBV DNA含量较高。
Objective To explore the clinical value of determining serum HBV DNA concentration in diagnosis and treatment of patients with hepatitis. Methods Serum HBV DNA of 568 patients with hepatitis B were determined by fluorescent quantitation polymerase chain reaction （FQ-PCR）. The differences of HBV DNA concentration among acute hepatitis （AH）, chromis hepatitis （CH） and liver cirrhosis （LC） were analyzed, as the HBV-M was examined. Results The concentration of serum HBV DNA in patients with AH（2.62 ± 1.73 copies/ml） was significantly lower than that of CH（5.68 ± 1.98 copies/ml）（ P = 0.007）and LC （4.87 ± 1.82 copies/ml） （P = 0.031 ）. There was no significant difference between serum HBV DNA of CH and LC （ P = 0.093）. The total concentration of HBV DNA in positive-HBeAg patients（5.83 ± 1.42 copies/ ml） was significantly higher than that of negative- HBeAg patients （ 1.88 ± 1.02 copies /ml） （ P = 0. 000）. Conclusion The concentration of serum HBV DNA is closely related with the course of hepatitis B and it is higher in positive-HBeAg patients.
出处
《河南职工医学院学报》
2006年第5期391-393,共3页
Journal of Henan Medical College For Staff and Workers
关键词
乙型肝炎病毒
DNA
荧光定量聚合酶链反应
Hepatitis B virus
DNA
fluorescent quantitation polymerase chain reaction
