摘要
背景与目的研究α-生育酚琥珀酸酯(α-Tocopherylsuccinate,α-TOS)抑制实验动物体内肿瘤的生长作用,并探讨其作用机制。材料与方法建立Ehrlich氏腹水癌小鼠实验动物模型,设阴性对照组、肿瘤模型对照组、α-TOS实验组(20、40和80mg/kg)和环磷酰胺对照组,观察α-TOS对Ehrlich氏腹水癌小鼠生存期的延长作用,检测各实验组动物血液学指标、NK细胞活性、T细胞亚群,应用DNA琼脂糖凝胶电泳检测α-TOS诱导肿瘤细胞凋亡的作用。结果80mg/kg剂量组α-TOS对Ehrlich氏腹水癌小鼠的生命延长率达33.09%;α-TOS各实验组白细胞(WBC)含量明显高于环磷酰胺阳性对照组,血小板(PLT)含量低于瘤模型对照组;40和80mg/kgα-TOS两剂量组NK细胞活性明显高于肿瘤模型对照组(P<0.05,P<0.01);α-TOS各实验组T细胞亚群数值接近于阴性对照组;在80mg/kg剂量下α-TOS处理的Ehrlich氏腹水癌小鼠腹水细胞中检测到明显的DNA梯度(Ladder)。结论α-TOS可延长Ehrlich氏腹水癌小鼠的生存时间,减轻荷瘤小鼠因血小板增多引起的血液粘稠状态,且无降低白细胞的副作用;增强荷瘤小鼠NK细胞杀伤活性、纠正T淋巴细胞亚群异常,缓解和改善荷瘤小鼠免疫力低下和免疫紊乱状态,激发机体免疫系统并在抑制肿瘤生长过程中发挥作用,并且α-TOS可诱导Ehrlich氏腹水癌小鼠腹水癌细胞凋亡。
BACKGROUND ＆ Ehrlich ascitesocarcinoma in vivo AIM： To study the inhibiting and explore the mechanisms. effects of α-tocopheryl succinate（α-TOS） on the growth of MATERIAL AND METHODS： The mice loaded with Ehrlich ascitesocarcinoma were used as tumor model, all the experimental animals were divided into negative control, tumor model, α-TOS treatment at dose 20, 40 and 80 mg/kg and cyclophosphamide positive groups. The survival ratio of tumor-beating mice and subsequent hematological changes were measured, NK cytotoxicity and the proportion of T lymphocyte subsets were detected by lactate dehydrogenase method and Flow Cytometer, respectively. Inducibility of Ehrlich cell apoptosis was examined by DNA agarose gel electrophoresis. RESULTS： The survival ratio in α-TOS treatment group at dose 80 mg/ kg was 33.09%. The white blood cell counts in α-TOS treatment groups were much higher than in positive control, the platelet counts were much lower than in tumor model. The NK cytotoxicity became much higher in α-TOS treatment groups at dose 40 and 80 rag/ kg compared with the tumor model（P 〈 0.05 and P 〈 0.01）. The T lymphocyte subset counts were close to negative control in α-TOS treatment groups. The DNA ladder was found in Ehrlich ascitesocarcinoma cells at dose 80 mg/ kg. CONCLUSION： α-TOS was capable of prolonging the survival time of mice with Ehrlich ascitesocarcinoma; could alleviate hyperviscosity caused by the increase in the nmnber of platelets, without interfering with the nmnber of leucocytes; was able to activate immune system in the process of inhibiting the growth of tumor due to improved immune function. The result of DNA agarose gel electmphoresis demonstrated that α-TOS induced apoptosis of Ehrlich ascitesocareinoma cells at a α-Tocopheryl succinate ; Ehrlich ascitesocarcinoma; antineoplastic dose of effect 80mg/kg
出处
《癌变．畸变．突变》
CAS
CSCD
2006年第5期370-373,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
黑龙江科技厅科技攻关项目(GB01C105)
