摘要
目的:观察Caspase-3抑制剂Z-DEVD-FMK对大鼠脊髓损伤后细胞凋亡的影响。方法:将36只SD大鼠以改良Allen法制作大鼠急性脊髓损伤(SCI)模型(脊髓中度损伤),分为对照组(单纯损伤A组18只,按时间段又分为A1,A2,A3),治疗组(应用Z-DEVD-FMK治疗B组18只,按时间段又分为B1,B2,B3)。损伤后6h,3d,8d对脊髓损伤区进行组织学HE染色,细胞凋亡TUNEL标记,Fas凋亡因子检测,免疫组化检测Caspase-3的表达变化,同时采用BBB评分方法和斜板试验观察神经功能恢复情况。结果:对照组和治疗组均见TUNEL阳性的凋亡细胞和Cas-pase-3表达。Z-DEVD-FMK治疗组神经细胞的凋亡数减少,Caspase-3表达降低,神经功能增强,两组相比差异有显著性(P<0.05)。结论:Caspase-3抑制剂Z-DEVD-FMK能减少继发性脊髓损伤细胞凋亡和促进神经功能的恢复。
Objective： To investigate the effect of Caspase-3 inhibitor Z-DEVD-FMK on neural cell apoptosis after spinal cord injury in rats. Method： The models of acute spinal cord injury in 36 SD rats were made with the improved Allen＇s method to get middle-degree injured. The rats were divided into two groups including control group （injury without treatment）, cured group （injury with Z-DEVD-FMK cured）. The treated rats were executed at 6h, 3d and 8d. The samples were examined with HE dyeing, immunocytochemistry technique fas and TUNEL dyeing and the expression of Caspase-3 by immunohistochemistry. The neurofuntion of spinal cord was measured by BBB score and Slope plate experiment.Result： The apoptosis cells positive for TUNEL and Caspase-3 expression were detectable in both two groups.In the cured group,the number of apoptotic cells and the express of Caspase-3 were decreased anti the neurofunction of the spinal cord improved as compared with those in the control group. Conclusion： Caspase-3 inhibitor Z-DEVD-FMK can reduce the numbers of apoptotic cells and promote the nerve function recovery after spinal cord injury.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2006年第9期792-795,i0001,共5页
Chinese Journal of Rehabilitation Medicine
