摘要
BACKGROUND： The long-term neurotoxicity of 3,4-methylenedioxymethamphetamine （MDMA） mainly caused by repeated exposure to MDMA or a single big dose of MDMA, which results in degeneration of serotonin terminal of central nervous system, and someone believe that the great release of serotonin transmitter in central nervous system will lead to anxious mood. OBJECTIVE： To observe the changes of anxiety related behaviors in rats after single administration of different doses of MDMA. DESIGN： A randomized control study SETTING： Laboratory of Psychopharmacology of the Mental Health Center, West China Hospital of Sichuan University. MATERIALS： Thirty male adult Wistar rats, weighing （251.3＋18.34） g, were used, MDMA were obtained from the National Institute for the Control of Pharmaceutical and Biological Products, and dissolved in saline. All the doses of the drug were administered in a volume of 1 mg/kg. METHODS ： The experiment was carried out in the Laboratory of Psychopharmacology of the Mental Health Center, West China Hospital of Sichuan University in July 2003. ①The rats were randomly divided into control group （n=6） and experimental group （n=24）, and then those in the latter were randomly assigned into four subgroups of MDMA 3, 5, 10 and 20 mg/kg groups, with 6 rats in each, which were administrated by single intraperitoneal injection of MDMA 3, 5, 10 and 20 mg/kg respectively, and those in the control group were administrated by single intraperitoneal injection of saline of the same volume. ② The open field test, elevated plus-maze test and social interaction test were performed immediately after administration. For the open field test, the apparatus was situated in a darkened room, illuminated by a single 60 W white light bulb located approximately 60 cm above the center of the open field. Before administration, all the rats were placed into the open field to be familiar with the open field for 5 minutes. They were observed for 45 minutes after administration. The locomotion �
BACKGROUND： The long-term neurotoxicity of 3,4-methylenedioxymethamphetamine （MDMA） mainly caused by repeated exposure to MDMA or a single big dose of MDMA, which results in degeneration of serotonin terminal of central nervous system, and someone believe that the great release of serotonin transmitter in central nervous system will lead to anxious mood. OBJECTIVE： To observe the changes of anxiety related behaviors in rats after single administration of different doses of MDMA. DESIGN： A randomized control study SETTING： Laboratory of Psychopharmacology of the Mental Health Center, West China Hospital of Sichuan University. MATERIALS： Thirty male adult Wistar rats, weighing （251.3＋18.34） g, were used, MDMA were obtained from the National Institute for the Control of Pharmaceutical and Biological Products, and dissolved in saline. All the doses of the drug were administered in a volume of 1 mg/kg. METHODS ： The experiment was carried out in the Laboratory of Psychopharmacology of the Mental Health Center, West China Hospital of Sichuan University in July 2003. ①The rats were randomly divided into control group （n=6） and experimental group （n=24）, and then those in the latter were randomly assigned into four subgroups of MDMA 3, 5, 10 and 20 mg/kg groups, with 6 rats in each, which were administrated by single intraperitoneal injection of MDMA 3, 5, 10 and 20 mg/kg respectively, and those in the control group were administrated by single intraperitoneal injection of saline of the same volume. ② The open field test, elevated plus-maze test and social interaction test were performed immediately after administration. For the open field test, the apparatus was situated in a darkened room, illuminated by a single 60 W white light bulb located approximately 60 cm above the center of the open field. Before administration, all the rats were placed into the open field to be familiar with the open field for 5 minutes. They were observed for 45 minutes after administration. The locomotion �
