摘要
采用聚合酶链反应（ＰｏｌｙｍｅｒａｓｅＣｈａｉｎＲｅａｃｔｉｏｎ，ＰＣＲ）扩增肺炎支原体（Ｍｙｃｏｐｌａｓｍａｐｎｅｕｍｏｎｉａｅ，Ｍｐ）特异的１４４ｂｐＤＮＡ片段，并将ＰＣＲ扩增与常规培养或血清冷凝集素测定相比较。结果表明，该法的阳性率明显高于常规培养或冷凝集素测定。２５例患者标本常规培养阳性６例（２４．０％），ＰＣＲ扩增阳性１１例（４４．０％），５０例患儿咽拭子ＰＣＲ扩增阳性１５例（３０．０％），其中２７例同时进行了ＰＣＲ和血清冷凝集素测定，阳性率分别为４０．７％（１１／２７）和２５．９％（７／２７）。ＰＣＲ扩增阳性病例改用红霉素或四环素治疗均获显著疗效。本法具有快速、特异、灵敏度高等优点，可望成为早期诊断Ｍｐ感染的常规方法。
Detection of Mycoplasma pneumoniae(Mp)in clinical specimens by amplifying a specific fragment of 144 base pairs using Polymerase Chain Reaction(PCR) was compared with detection by culture or serum cold agglutination. The results showed that PCR was more sensitive than conventional culture techniques or cold agglutination for the detection of Mp.Of the 25 adult patients, positive results of Mp culture and PCR were 6(24.0%) and 11(44.0%),respectively. Of 50 infant patients, positive results of throat swabs of PCR were 15(30.0%).27 of the 50 patients were examined simultaneously by PCR and serum cold agglutination, positive results being 11(40.7%)and 7(25.9%),respectively.Significant effects were obtained after treatment of erythromycin or tetracycline in patients with positive Mp results by PCR.PCR is a rapid,sensitive and specific method for the detection of Mp infected clinical specimens.
出处
《同济医科大学学报》
CSCD
北大核心
1996年第5期370-372,共3页
Acta Universitatis Medicinae Tongji
