摘要
目的：探讨脊髓损伤继发骨质疏松的理论依据以指导临床治疗。方法：雄性Wistar大鼠120只随机分12组（每组10只），各对照组分别行胸10椎板切除，不损伤硬膜及脊髓后0、1、2、3、7和11周，实验组分别于胸10椎板切除后行Allen＇s法（60gcm）损伤脊髓（导致截瘫）后0、1、2、3、7和11周，检测血钙（Ca）、血磷（P）、血清碱性磷酸酶（ALP）及肱骨外科颈部、股骨粗隆部、胫骨平台部松质骨骨密度（BMD）改变情况。结果：实验组（ALP术后1及3周低于对照组（P＜0．05）；7周时明显高于对照组（P＜0．01）；实验组血磷（P）术后1、2周高于对照组（P＜0．01），实验组血钙（Ca）术后1～3周时均明显高于对照组（P＜0．01），然后逐渐降至略高于正常。实验组肱骨BMD术后3、7及11周时均明显低于对照组（P＜0．01），以3周时最低；实验组股骨BMD术后7周时低于对照组（P＜0．05），11周时与对照组比较差异无显著性（P〉0．05）；实验组胫骨BMD术后7及11周时均明显低于对照组（P＜0．01）实验组术后7与11周比较差异无显著性（P〉0．05）。结论：大鼠脊髓损伤截瘫后血液生化指标改变，损伤平面上下均继发骨质疏松，损伤平面上骨密度恢复较早。
Objective To study the changes of biochemistry indexes in blood serum and bone mineral density （BMD） of secondary osteoporosis after spinal cord injury （SCI） in rats. Methods 120 male Wistar rats were divided randomly into 12 groups （n= 10）. The rats in control groups were only removed larmina with dura intact, without spinal cord injury at the level of the tenth thoracic vertebrae, the rats in experimental groups were also removed larmina, then paraplegic model was made by the method of Allen＇s （60gcm）. The changes of biochemistry indexes in blood serum and BMD were observed. Results Compared with control groups, the concentrations of alkaline phophatase （ALP） were decreased at the 1st and the 3rd week in experimental groups （P〈0.05）, increased significantly at the 7th week in experimental groups （P〈0.01）. the concentrations of phosphorus were increased at the 1st and the 2nd week in experimental groups （P〈0.01） ; the concentrations of calcium were increased at the 1st .2nd and 3rd week in experimental groups （P〈0.01） ; then the concentrations of calcium and phosphorus declined to a little higher than normal. Compared with control group, the BMD of humerus were decreased at the 3rd, 7th and 11th week in experimental group （P〈0.01）, especially at the 3rd week; BMD of femur was decreased at the 7th week in experimental group （P〈0.01）, there was no difference between the 11th week group and control group （P〈0.05）. BMD of tibia was decreased at the 7th and 11th week in experimental group （P〈0.01）, but there was no difference between the 7th and the 11th week in experimental groups. Conclusion The changes of biochemistry indexes in blood serum fluctuated after spinal cord injury can be found , osteoporosis is found in bones both superlesion and sublesion, and bone superlesion rehabilitate early in paraplegic rats.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2006年第5期835-838,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅自然科学基金项目资助课题(2005051664)
